Cytokeratin 20 MRNA in Peripheral Venous Blood of Colorectal Carcinoma Patients

Overview

Journal Br J Cancer

Specialty Oncology

Date 1998 May 14

PMID 9579841

Citations 21

Authors

N O Funaki

J Tanaka

G Ohshio

H Onodera

S Maetani

M Imamura

Affiliations

Soon will be listed here.

Abstract

A highly sensitive system was previously developed by us to detect the presence of colorectal carcinoma cells in blood in the form of cytokeratin 20 (CK20) mRNA. In the present study, we used an improved version of this system to analyse the peripheral blood of 28 patients with colorectal carcinoma, five patients with non-cancerous intestinal diseases and six normal controls for the presence or absence of CK20 mRNA and to investigate the relationship between the mRNA results and prognosis. All eight patients with recurrence were positive for CK20 mRNA, as were four patients in the Dukes' C stage with either distant metastasis or dissemination. Five of the nine patients in the Dukes' C stage with neither distant metastasis nor dissemination were positive, and three of these developed recurrence within 11 months after the analysis. Only one of the seven patients in the Dukes' A or B stage was positive, and none showed recurrence during the 1-19 months of observation. None of the five patients without carcinomas or of the six normal controls was positive. Although the follow-up period is limited and the recurrences were all local at present, these results suggest that the presence of CK20 mRNA in circulation may be a useful indicator for the screening of advanced colorectal carcinoma patients with a high risk of recurrence.

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References

Semple T, Quinn L, Woods L, Moore G . Tumor and lymphoid cell lines from a patient with carcinoma of the colon for a cytotoxicity model. Cancer Res. 1978; 38(5):1345-55. View

Taylor I . Liver metastases from colorectal cancer: lessons from past and present clinical studies. Br J Surg. 1996; 83(4):456-60. DOI: 10.1002/bjs.1800830406. View

Pearlman N, Donohue R, Stiegmann G, Ahnen D, Sedlacek S, Braun T . Pelvic and sacropelvic exenteration for locally advanced or recurrent anorectal cancer. Arch Surg. 1987; 122(5):537-41. DOI: 10.1001/archsurg.1987.01400170043006. View

Chomczynski P, Sacchi N . Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162(1):156-9. DOI: 10.1006/abio.1987.9999. View

Fuqua S, Falette N, McGuire W . Sensitive detection of estrogen receptor RNA by polymerase chain reaction assay. J Natl Cancer Inst. 1990; 82(10):858-61. DOI: 10.1093/jnci/82.10.858. View

Moll R, Lowe A, Laufer J, Franke W . Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol. 1992; 140(2):427-47. PMC: 1886432. View

Mayer B, Johnson J, Leitl F, Jauch K, Heiss M, Schildberg F . E-cadherin expression in primary and metastatic gastric cancer: down-regulation correlates with cellular dedifferentiation and glandular disintegration. Cancer Res. 1993; 53(7):1690-5. View

Moll R, Zimbelmann R, Goldschmidt M, Keith M, Laufer J, Kasper M . The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 1993; 53(2):75-93. DOI: 10.1111/j.1432-0436.1993.tb00648.x. View

Mayer B, Jauch K, Gunthert U, Figdor C, Schildberg F, Funke I . De-novo expression of CD44 and survival in gastric cancer. Lancet. 1993; 342(8878):1019-22. DOI: 10.1016/0140-6736(93)92879-x. View

10.

Gillespie D, Cuddy K, Kolbe T, Marks D . Dissolve and capture: a strategy for analysing mRNA in blood. Nature. 1994; 367(6461):390-1. DOI: 10.1038/367390a0. View

11.

Burchill S, Bradbury M, Pittman K, Southgate J, Smith B, Selby P . Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Br J Cancer. 1995; 71(2):278-81. PMC: 2033587. DOI: 10.1038/bjc.1995.56. View

12.

Togo S, Shimada H, Kubota T, Moossa A, Hoffman R . "Seed" to "soil" is a return trip in metastasis. Anticancer Res. 1995; 15(3):791-4. View

13.

Funaki N, Tanaka J, Seto S, Kasamatsu T, Kaido T, Imamura M . Highly-sensitive identification of alpha-fetoprotein mRNA in circulating peripheral blood of hepatocellular carcinoma patients. Life Sci. 1995; 57(17):1621-31. DOI: 10.1016/0024-3205(95)02138-9. View

14.

Funaki N, Tanaka J, Kasamatsu T, Ohshio G, Hosotani R, Okino T . Identification of carcinoembryonic antigen mRNA in circulating peripheral blood of pancreatic carcinoma and gastric carcinoma patients. Life Sci. 1996; 59(25-26):2187-99. DOI: 10.1016/s0024-3205(96)00576-0. View

15.

Funaki N, Tanaka J, Itami A, Kasamatsu T, Ohshio G, Onodera H . Detection of colorectal carcinoma cells in circulating peripheral blood by reverse transcription-polymerase chain reaction targeting cytokeratin-20 mRNA. Life Sci. 1997; 60(9):643-52. DOI: 10.1016/s0024-3205(96)00700-x. View

16.

Funaki N, Tanaka J, Seto S, Kasamatsu T, Kaido T, Imamura M . Hematogenous spreading of hepatocellular carcinoma cells: possible participation in recurrence in the liver. Hepatology. 1997; 25(3):564-8. DOI: 10.1002/hep.510250312. View

17.

Moll R, Franke W, Schiller D, Geiger B, Krepler R . The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982; 31(1):11-24. DOI: 10.1016/0092-8674(82)90400-7. View