Hematogenous Tumor Cell Dissemination During Colonoscopy for Colorectal Cancer

Overview

Journal Surg Endosc

Publisher Springer

Specialties Gastroenterology
General Surgery
Radiology

Date 2004 Jan 22

PMID 14735340

Citations 21

Authors

M Koch

P Kienle

P Sauer

F Willeke

K Buhl

A Benner

T Lehnert

C Herfarth

M von Knebel Doeberitz

J Weitz

Affiliations

Soon will be listed here.

Abstract

Background: It has long been suspected that mechanical influences may enhance the release of viable colorectal cancer cells into the circulation. The objective of this study was to determine the extent of hematogenous tumor cell spread in colorectal cancer patients during colonoscopy.

Methods: Peripheral venous blood samples were taken before and after colonoscopy from 44 patients with colorectal cancer. Blood samples were examined using a reverse-transcriptase polymerase chain reaction assay to amplify cytokeratin 20 transcripts.

Results: Eleven patients with colorectal cancer displayed circulating tumor cells before and after colonoscopy (25%), whereas tumor cells were detected in six of 44 patients (14%) only after the procedure (p = 0.03, McNemar's test: tumor cell detection before after colonoscopy). All control samples consistently tested negative.

Conclusions: Mechanical forces may result in enhanced release of viable colorectal cancer cells into the circulation; however, the clinical significance of these results needs to be clarified.

Citing Articles

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Iguchi K, Mushiake H, Aoyama T, Suwa H, Yukawa N, Ota M In Vivo. 2019; 33(4):1243-1248.

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References

Ghossein R, Bhattacharya S, Rosai J . Molecular detection of micrometastases and circulating tumor cells in solid tumors. Clin Cancer Res. 1999; 5(8):1950-60. View

Wyld D, Selby P, Perren T, Jonas S, Allen-Mersh T, Wheeldon J . Detection of colorectal cancer cells in peripheral blood by reverse-transcriptase polymerase chain reaction for cytokeratin 20. Int J Cancer. 1998; 79(3):288-93. DOI: 10.1002/(sici)1097-0215(19980619)79:3<288::aid-ijc14>3.0.co;2-4. View

Burchill S, Bradbury M, Pittman K, Southgate J, Smith B, Selby P . Detection of epithelial cancer cells in peripheral blood by reverse transcriptase-polymerase chain reaction. Br J Cancer. 1995; 71(2):278-81. PMC: 2033587. DOI: 10.1038/bjc.1995.56. View

Turner J, Sturgeon C . Effect of bowel cleansing and colonoscopy on serum CEA levels. J R Coll Surg Edinb. 1993; 38(2):89-91. View

Macfarlane D, Dahle C . Isolating RNA from whole blood--the dawn of RNA-based diagnosis?. Nature. 1993; 362(6416):186-8. DOI: 10.1038/362186a0. View

Wiggers T, Jeekel J, Arends J, BRINKHORST A, Kluck H, Luyk C . No-touch isolation technique in colon cancer: a controlled prospective trial. Br J Surg. 1988; 75(5):409-15. DOI: 10.1002/bjs.1800750505. View

Moll R, Zimbelmann R, Goldschmidt M, Keith M, Laufer J, Kasper M . The human gene encoding cytokeratin 20 and its expression during fetal development and in gastrointestinal carcinomas. Differentiation. 1993; 53(2):75-93. DOI: 10.1111/j.1432-0436.1993.tb00648.x. View

Weitz J, Koch M, Kienle P, Schrodel A, Willeke F, Benner A . Detection of hematogenic tumor cell dissemination in patients undergoing resection of liver metastases of colorectal cancer. Ann Surg. 2000; 232(1):66-72. PMC: 1421109. DOI: 10.1097/00000658-200007000-00010. View

Nishizaki T, Matsumata T, Kanematsu T, Yasunaga C, Sugimachi K . Surgical manipulation of VX2 carcinoma in the rabbit liver evokes enhancement of metastasis. J Surg Res. 1990; 49(1):92-7. DOI: 10.1016/0022-4804(90)90116-j. View

10.

Moll R, Lowe A, Laufer J, Franke W . Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol. 1992; 140(2):427-47. PMC: 1886432. View

11.

Pappalardo G, Polettini E, Frattaroli F, Casciani E, DOrta C, Damato M . Magnetic resonance colonography versus conventional colonoscopy for the detection of colonic endoluminal lesions. Gastroenterology. 2000; 119(2):300-4. DOI: 10.1053/gast.2000.9353. View

12.

Moreno J, OHara S, Long J, Veltri R, Ning X, Alexander A . Transrectal ultrasound-guided biopsy causes hematogenous dissemination of prostate cells as determined by RT-PCR. Urology. 1997; 49(4):515-20. DOI: 10.1016/s0090-4295(97)00016-2. View

13.

Willeke F, Ridder R, Mechtersheimer G, Schwarzbach M, Duwe A, Weitz J . Analysis of FUS-CHOP fusion transcripts in different types of soft tissue liposarcoma and their diagnostic implications. Clin Cancer Res. 1998; 4(7):1779-84. View

14.

Allgayer H, Heiss M, Riesenberg R, Grutzner K, Tarabichi A, Babic R . Urokinase plasminogen activator receptor (uPA-R): one potential characteristic of metastatic phenotypes in minimal residual tumor disease. Cancer Res. 1997; 57(7):1394-9. View

15.

Howard M, Greene F . The effect of preoperative endoscopy on recurrence and survival following surgery for colorectal carcinoma. Am Surg. 1990; 56(3):124-7. View

16.

Rex D . CT and MR colography (virtual colonoscopy): status report. J Clin Gastroenterol. 1998; 27(3):199-203. DOI: 10.1097/00004836-199810000-00004. View

17.

Yuan J, Coplen D, Petros J, Figenshau R, Ratliff T, Smith D . Effects of rectal examination, prostatic massage, ultrasonography and needle biopsy on serum prostate specific antigen levels. J Urol. 1992; 147(3 Pt 2):810-4. DOI: 10.1016/s0022-5347(17)37392-5. View

18.

Sollenberger L, Eisenstat T, RUBIN R, SALVATI E . Is preoperative colonoscopy necessary in carcinoma of the colon and rectum?. Am Surg. 1988; 54(2):113-5. View

19.

Jung R, Petersen K, Kruger W, Wolf M, Wagener C, Zander A . Detection of micrometastasis by cytokeratin 20 RT-PCR is limited due to stable background transcription in granulocytes. Br J Cancer. 1999; 81(5):870-3. PMC: 2374287. DOI: 10.1038/sj.bjc.6690778. View

20.

Basha G, Ectors N, Penninckx F, Filez L, Geboes K . Tumor cell implantation after colonoscopy with biopsies in a patient with rectal cancer: report of a case. Dis Colon Rectum. 1998; 40(12):1508-10. DOI: 10.1007/BF02070721. View