Incorporation of Fowl Plague Virus Hemagglutinin into Murine Leukemia Virus Particles and Analysis of the Infectivity of the Pseudotyped Retroviruses

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1998 May 30

PMID 9573311

Citations 12

Authors

T Hatziioannou

S Valsesia-Wittmann

S J Russell

F L Cosset

Affiliations

Soon will be listed here.

Abstract

We describe retrovirus particles carrying the fowl plague virus (FPV) hemagglutinin (HA). When expressed in cells providing Moloney murine leukemia virus (MoMLV) Gag and Pol proteins and a lacZ retroviral vector, FPV HA was found to be efficiently expressed, correctly processed, and stably incorporated into retroviral particles. HA-bearing retroviruses were infectious with a wide host range and were only 10-fold less infectious than retroviruses carrying wild-type MLV retroviral envelopes. We also coexpressed HA proteins in retroviral particles with chimeric MoMLV-derived envelope glycoproteins that efficiently retarget virus attachment but are only weakly fusogenic. Our results suggest that HA can in some cases enhance the fusion ability of these retroviral particles, depending on the cell surface molecule that is used as a receptor.

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References

Wilson I, Skehel J, Wiley D . Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution. Nature. 1981; 289(5796):366-73. DOI: 10.1038/289366a0. View

Cosset F, Russell S . Targeting retrovirus entry. Gene Ther. 1996; 3(11):946-56. View

Wiley D, Skehel J . The structure and function of the hemagglutinin membrane glycoprotein of influenza virus. Annu Rev Biochem. 1987; 56:365-94. DOI: 10.1146/annurev.bi.56.070187.002053. View

Marsh M, Helenius A . Virus entry into animal cells. Adv Virus Res. 1989; 36:107-51. PMC: 7130955. DOI: 10.1016/s0065-3527(08)60583-7. View

Chen W, LAZAR C, Lund K, Welsh J, Chang C, Walton G . Functional independence of the epidermal growth factor receptor from a domain required for ligand-induced internalization and calcium regulation. Cell. 1989; 59(1):33-43. DOI: 10.1016/0092-8674(89)90867-2. View

Evans L, Morrison R, Malik F, Portis J, Britt W . A neutralizable epitope common to the envelope glycoproteins of ecotropic, polytropic, xenotropic, and amphotropic murine leukemia viruses. J Virol. 1990; 64(12):6176-83. PMC: 248792. DOI: 10.1128/JVI.64.12.6176-6183.1990. View

Dong J, Roth M, Hunter E . A chimeric avian retrovirus containing the influenza virus hemagglutinin gene has an expanded host range. J Virol. 1992; 66(12):7374-82. PMC: 240443. DOI: 10.1128/JVI.66.12.7374-7382.1992. View

White J . Membrane fusion. Science. 1992; 258(5084):917-24. DOI: 10.1126/science.1439803. View

Ohuchi M, Cramer A, Vey M, Ohuchi R, Garten W, Klenk H . Rescue of vector-expressed fowl plague virus hemagglutinin in biologically active form by acidotropic agents and coexpressed M2 protein. J Virol. 1994; 68(2):920-6. PMC: 236529. DOI: 10.1128/JVI.68.2.920-926.1994. View

10.

Bullough P, Hughson F, Skehel J, Wiley D . Structure of influenza haemagglutinin at the pH of membrane fusion. Nature. 1994; 371(6492):37-43. DOI: 10.1038/371037a0. View

11.

Cosset F, Morling F, Takeuchi Y, Weiss R, Collins M, Russell S . Retroviral retargeting by envelopes expressing an N-terminal binding domain. J Virol. 1995; 69(10):6314-22. PMC: 189530. DOI: 10.1128/JVI.69.10.6314-6322.1995. View

12.

Ohuchi M, Feldmann A, Ohuchi R, Klenk H . Neuraminidase is essential for fowl plague virus hemagglutinin to show hemagglutinating activity. Virology. 1995; 212(1):77-83. DOI: 10.1006/viro.1995.1455. View

13.

Cosset F, Takeuchi Y, Battini J, Weiss R, Collins M . High-titer packaging cells producing recombinant retroviruses resistant to human serum. J Virol. 1995; 69(12):7430-6. PMC: 189680. DOI: 10.1128/JVI.69.12.7430-7436.1995. View

14.

Valsesia-Wittmann S, Morling F, Nilson B, Takeuchi Y, Russell S, Cosset F . Improvement of retroviral retargeting by using amino acid spacers between an additional binding domain and the N terminus of Moloney murine leukemia virus SU. J Virol. 1996; 70(3):2059-64. PMC: 190040. DOI: 10.1128/JVI.70.3.2059-2064.1996. View

15.

Ikuta S, Yamada Y, Yoshitake Y, Nishikawa K . Production and properties of a monoclonal antibody against human epidermal growth factor. Biochem Int. 1985; 10(2):251-8. View