High-titer Packaging Cells Producing Recombinant Retroviruses Resistant to Human Serum

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1995 Dec 1

PMID 7494248

Citations 167

Authors

F L Cosset

Y Takeuchi

J L Battini

R A Weiss

M K Collins

Affiliations

Soon will be listed here.

Abstract

Novel retroviral protein expression constructs were designed to retain minimal retroviral sequences and to express dominant selectable markers by reinitiation of translation after expression of the viral genes. HT1080 cells were selected as producer cells for their ability to release high-titer viruses that are resistant to inactivation by human serum. Two HT1080-based packaging cell lines which produce Moloney murine leukemia virus cores with envelope glycoproteins of either amphotropic murine leukemia virus (FLYA13 line) or cat endogenous virus RD114 (FLYRD18 line) are described. Direct comparison with previous retroviral packaging systems indicated that 100-fold-higher titers of helper-free recombinant viruses were released by the FLYA13 and FLYRD18 lines.

Citing Articles

Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors.

van Heuvel Y, Schatz S, Hein M, Dogra T, Kazenmaier D, Tschorn N Front Bioeng Biotechnol. 2023; 11:1076524.

PMID: 37082212 PMC: 10112512. DOI: 10.3389/fbioe.2023.1076524.

Efficient clinical-grade γ-retroviral vector purification by high-speed centrifugation for CAR T cell manufacturing.

Mekkaoui L, Tejerizo J, Abreu S, Rubat L, Nikoniuk A, Macmorland W Mol Ther Methods Clin Dev. 2023; 28:116-128.

PMID: 36620071 PMC: 9808014. DOI: 10.1016/j.omtm.2022.12.006.

IDO1, FAT10, IFI6, and GILT Are Involved in the Antiretroviral Activity of γ-Interferon and IDO1 Restricts Retrovirus Infection by Autophagy Enhancement.

Kubo Y, Yasui K, Izumida M, Hayashi H, Matsuyama T Cells. 2022; 11(14).

PMID: 35883685 PMC: 9323257. DOI: 10.3390/cells11142240.

Lentiviral Vector Bioprocessing.

Perry C, Rayat A Viruses. 2021; 13(2).

PMID: 33572347 PMC: 7916122. DOI: 10.3390/v13020268.

A clinically applicable and scalable method to regenerate T-cells from iPSCs for off-the-shelf T-cell immunotherapy.

Iriguchi S, Yasui Y, Kawai Y, Arima S, Kunitomo M, Sato T Nat Commun. 2021; 12(1):430.

PMID: 33462228 PMC: 7814014. DOI: 10.1038/s41467-020-20658-3.

References

Scarpa M, Cournoyer D, Muzny D, Moore K, Belmont J, Caskey C . Characterization of recombinant helper retroviruses from Moloney-based vectors in ecotropic and amphotropic packaging cell lines. Virology. 1991; 180(2):849-52. DOI: 10.1016/0042-6822(91)90105-k. View

Girod A, Cosset F, Verdier G, Ronfort C . Analysis of ALV-based packaging cell lines for production of contaminant defective viruses. Virology. 1995; 209(2):671-5. DOI: 10.1006/viro.1995.1302. View

Miller A, Garcia J, von Suhr N, Lynch C, Wilson C, Eiden M . Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J Virol. 1991; 65(5):2220-4. PMC: 240569. DOI: 10.1128/JVI.65.5.2220-2224.1991. View

Cosset F, Legras C, Thomas J, Molina R, Chebloune Y, Faure C . Improvement of avian leukosis virus (ALV)-based retrovirus vectors by using different cis-acting sequences from ALVs. J Virol. 1991; 65(6):3388-94. PMC: 241003. DOI: 10.1128/JVI.65.6.3388-3394.1991. View

Heard J, Danos O . An amino-terminal fragment of the Friend murine leukemia virus envelope glycoprotein binds the ecotropic receptor. J Virol. 1991; 65(8):4026-32. PMC: 248833. DOI: 10.1128/JVI.65.8.4026-4032.1991. View

Izumi M, Miyazawa H, Kamakura T, Yamaguchi I, Endo T, Hanaoka F . Blasticidin S-resistance gene (bsr): a novel selectable marker for mammalian cells. Exp Cell Res. 1991; 197(2):229-33. DOI: 10.1016/0014-4827(91)90427-v. View

Perryman S, Nishio J, Chesebro B . Complete nucleotide sequence of Friend murine leukemia virus, strain FB29. Nucleic Acids Res. 1991; 19(24):6950. PMC: 329334. DOI: 10.1093/nar/19.24.6950. View

Battini J, Heard J, Danos O . Receptor choice determinants in the envelope glycoproteins of amphotropic, xenotropic, and polytropic murine leukemia viruses. J Virol. 1992; 66(3):1468-75. PMC: 240871. DOI: 10.1128/JVI.66.3.1468-1475.1992. View

Benveniste R, Todaro G . Homology between type-C viruses of various species as determined by molecular hybridization. Proc Natl Acad Sci U S A. 1973; 70(12):3316-20. PMC: 427227. DOI: 10.1073/pnas.70.12.3316. View

10.

Shinnick T, Lerner R, Sutcliffe J . Nucleotide sequence of Moloney murine leukaemia virus. Nature. 1981; 293(5833):543-8. DOI: 10.1038/293543a0. View

11.

Reeves R, OBrien S . Molecular genetic characterization of the RD-114 gene family of endogenous feline retroviral sequences. J Virol. 1984; 52(1):164-71. PMC: 254502. DOI: 10.1128/JVI.52.1.164-171.1984. View

12.

Kozak M . Effects of intercistronic length on the efficiency of reinitiation by eucaryotic ribosomes. Mol Cell Biol. 1987; 7(10):3438-45. PMC: 367994. DOI: 10.1128/mcb.7.10.3438-3445.1987. View

13.

Gatignol A, Durand H, Tiraby G . Bleomycin resistance conferred by a drug-binding protein. FEBS Lett. 1988; 230(1-2):171-5. DOI: 10.1016/0014-5793(88)80665-3. View

14.

Bestwick R, Kozak S, Kabat D . Overcoming interference to retroviral superinfection results in amplified expression and transmission of cloned genes. Proc Natl Acad Sci U S A. 1988; 85(15):5404-8. PMC: 281765. DOI: 10.1073/pnas.85.15.5404. View

15.

Danos O, Mulligan R . Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges. Proc Natl Acad Sci U S A. 1988; 85(17):6460-4. PMC: 281992. DOI: 10.1073/pnas.85.17.6460. View

16.

Shih A, Misra R, Rush M . Detection of multiple, novel reverse transcriptase coding sequences in human nucleic acids: relation to primate retroviruses. J Virol. 1989; 63(1):64-75. PMC: 247658. DOI: 10.1128/JVI.63.1.64-75.1989. View

17.

Markowitz D, Goff S, Bank A . Construction and use of a safe and efficient amphotropic packaging cell line. Virology. 1988; 167(2):400-6. View

18.

Scadden D, Fuller B, Cunningham J . Human cells infected with retrovirus vectors acquire an endogenous murine provirus. J Virol. 1990; 64(1):424-7. PMC: 249120. DOI: 10.1128/JVI.64.1.424-427.1990. View

19.

Ott D, Friedrich R, Rein A . Sequence analysis of amphotropic and 10A1 murine leukemia viruses: close relationship to mink cell focus-inducing viruses. J Virol. 1990; 64(2):757-66. PMC: 249170. DOI: 10.1128/JVI.64.2.757-766.1990. View

20.

Cosset F, Legras C, Chebloune Y, Savatier P, Thoraval P, Thomas J . A new avian leukosis virus-based packaging cell line that uses two separate transcomplementing helper genomes. J Virol. 1990; 64(3):1070-8. PMC: 249219. DOI: 10.1128/JVI.64.3.1070-1078.1990. View