Development and Applications of Enhanced Green Fluorescent Protein Mutants

Overview

Journal Biotechniques

Specialties Biomedical Engineering
Biotechnology

Date 1998 Apr 4

PMID 9526659

Citations 43

Authors

R H Stauber

K Horie

P Carney

E A Hudson

N I Tarasova

G A Gaitanaris

G N Pavlakis

Affiliations

Soon will be listed here.

Abstract

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

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