Over-expression of Testis-specific Expressed Gene 1 Attenuates the Proliferation and Induces Apoptosis of GC-1spg Cells

Overview

Journal J Huazhong Univ Sci Technolog Med Sci

Specialty General Medicine

Date 2014 Aug 20

PMID 25135723

Citations 1

Authors

Chao-Hui Gu

Feng-Yan Tian

Jia-Rui Pu

Li-Duan Zheng

Hong Mei

Fu-Qing Zeng

Jin-Jian Yang

Quan-Cheng Kan

Qiang-Song Tong

Affiliations

Soon will be listed here.

Abstract

The effects of over-expression of testis-specific expressed gene 1 (TSEG-1) on the viability and apoptosis of cultured spermatogonial GC-1spg cells were investigated, and the immortal spermatogonial cell line GC-1spg (CRL-2053™) was obtained as the cell model in order to explore the function of TSEG-1. We transfected the eukaryotic vector of TSEG-1, named as pEGFP-TSEG-1 into cultured spermatogonial GC-1spg cells. Over-expression of TSEG-1 inhibited the proliferation of GC-1spg cells, and arrested cell cycle slightly at G0/G1 phase. Transfection of TSEG-1 attenuated the transcript levels of Ki-67, PCNA and cyclin D1. In addition, over-expression of TSEG-1 induced early and late apoptosis, and reduced the mitochondrial membrane potential of GC-1spg cells. Moreover, transfection of TSEG-1 significantly enhanced the ratio of Bax/Bcl-2 and transcript levels of caspase 9, and decreased the expression of Fas and caspase 8 in GC-1spg cells. These results indicated over-expression of TSEG-1 suppresses the proliferation and induces the apoptosis of GC-1spg cells, which establishes a basis for further study on the function of TSEG-1.

Citing Articles

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References

Oldereid N, Angelis P, Wiger R, Clausen O . Expression of Bcl-2 family proteins and spontaneous apoptosis in normal human testis. Mol Hum Reprod. 2001; 7(5):403-8. DOI: 10.1093/molehr/7.5.403. View

Alavi S, Taghavi M, Moallem S . Evaluation of effects of methamphetamine repeated dosing on proliferation and apoptosis of rat germ cells. Syst Biol Reprod Med. 2008; 54(2):85-91. DOI: 10.1080/19396360801952078. View

Gu C, Tong Q, Zheng L, Liang Z, Pu J, Mei H . TSEG-1, a novel member of histone H2A variants, participates in spermatogenesis via promoting apoptosis of spermatogenic cells. Genomics. 2010; 95(5):278-89. DOI: 10.1016/j.ygeno.2010.02.005. View

Moreno R, Lizama C, Urzua N, Vergara S, Reyes J . Caspase activation throughout the first wave of spermatogenesis in the rat. Cell Tissue Res. 2006; 325(3):533-40. DOI: 10.1007/s00441-006-0186-4. View

Wright A, Reiley W, Chang M, Jin W, Lee A, Zhang M . Regulation of early wave of germ cell apoptosis and spermatogenesis by deubiquitinating enzyme CYLD. Dev Cell. 2007; 13(5):705-716. DOI: 10.1016/j.devcel.2007.09.007. View

Wu F, Caron C, De Robertis C, Khochbin S, Rousseaux S . Testis-specific histone variants H2AL1/2 rapidly disappear from paternal heterochromatin after fertilization. J Reprod Dev. 2008; 54(6):413-7. DOI: 10.1262/jrd.20067. View

Giampietri C, Petrungaro S, Coluccia P, DAlessio A, Starace D, Riccioli A . Germ cell apoptosis control during spermatogenesis. Contraception. 2005; 72(4):298-302. DOI: 10.1016/j.contraception.2005.04.011. View

Govin J, Escoffier E, Rousseaux S, Kuhn L, Ferro M, Thevenon J . Pericentric heterochromatin reprogramming by new histone variants during mouse spermiogenesis. J Cell Biol. 2007; 176(3):283-94. PMC: 2063955. DOI: 10.1083/jcb.200604141. View

Steger K, Aleithe I, Behre H, Bergmann M . The proliferation of spermatogonia in normal and pathological human seminiferous epithelium: an immunohistochemical study using monoclonal antibodies against Ki-67 protein and proliferating cell nuclear antigen. Mol Hum Reprod. 1998; 4(3):227-33. DOI: 10.1093/molehr/4.3.227. View

10.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

11.

Beumer T, Roepers-Gajadien H, Gademan I, Kal H, de Rooij D . Involvement of the D-type cyclins in germ cell proliferation and differentiation in the mouse. Biol Reprod. 2000; 63(6):1893-8. DOI: 10.1095/biolreprod63.6.1893. View

12.

Rodriguez I, Ody C, Araki K, Garcia I, Vassalli P . An early and massive wave of germinal cell apoptosis is required for the development of functional spermatogenesis. EMBO J. 1997; 16(9):2262-70. PMC: 1169828. DOI: 10.1093/emboj/16.9.2262. View

13.

Russell L, Chiarini-Garcia H, Korsmeyer S, Knudson C . Bax-dependent spermatogonia apoptosis is required for testicular development and spermatogenesis. Biol Reprod. 2002; 66(4):950-8. DOI: 10.1095/biolreprod66.4.950. View

14.

Gu C, Tong Q, Zeng F, Liu Y, Wang Z, Zheng L . [Cloning and sequence analysis of TSEG-1, a novel gene specifically expressed in mouse testis]. Yi Chuan. 2008; 30(3):352-8. DOI: 10.3724/sp.j.1005.2008.00352. View

15.

Boekelheide K . Mechanisms of toxic damage to spermatogenesis. J Natl Cancer Inst Monogr. 2005; (34):6-8. DOI: 10.1093/jncimonographs/lgi006. View

16.

Yazawa T, Yamamoto T, Nakayama Y, Hamada S, Abe S . Conversion from mitosis to meiosis: morphology and expression of proliferating cell nuclear antigen (PCNA) and Dmc1 during newt spermatogenesis. Dev Growth Differ. 2001; 42(6):603-11. DOI: 10.1046/j.1440-169x.2000.00544.x. View

17.

Stauber R, Horie K, Carney P, Hudson E, Tarasova N, Gaitanaris G . Development and applications of enhanced green fluorescent protein mutants. Biotechniques. 1998; 24(3):462-6, 468-71. DOI: 10.2144/98243rr01. View

18.

Yin Y, Stahl B, DeWolf W, Morgentaler A . P53 and Fas are sequential mechanisms of testicular germ cell apoptosis. J Androl. 2002; 23(1):64-70. DOI: 10.1002/jand.2002.23.1.64. View

19.

Sirianni R, Chimento A, Ruggiero C, De Luca A, Lappano R, Ando S . The novel estrogen receptor, G protein-coupled receptor 30, mediates the proliferative effects induced by 17beta-estradiol on mouse spermatogonial GC-1 cell line. Endocrinology. 2008; 149(10):5043-51. DOI: 10.1210/en.2007-1593. View

20.

Saradha B, Vaithinathan S, Mathur P . Lindane induces testicular apoptosis in adult Wistar rats through the involvement of Fas-FasL and mitochondria-dependent pathways. Toxicology. 2008; 255(3):131-9. DOI: 10.1016/j.tox.2008.10.016. View