The Polymeric Immunoglobulin Receptor (secretory Component) in a Human Intestinal Epithelial Cell Line is Up-regulated by Interleukin-1

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1998 Jan 1

PMID 9415030

Citations 19

Authors

M Hayashi

N Takenouchi

M Asano

M Kato

T Tsurumachi

T Saito

I Moro

Affiliations

Soon will be listed here.

Abstract

Secretory component (SC or polymeric immunoglobulin receptor) on mucosal epithelial cells mediates transcytosis of polymeric immunoglobulin into external fluids and functions as a receptor for polymeric immunoglobulin. SC expression in a human colonic adenocarcinoma cell line, HT-29 has been reported to be up-regulated by various cytokines, such as interferon-gamma, tumour necrosis factor-alpha and interleukin-4 (IL-4). However, up-regulation of SC by IL-1 is controversial. In this study, we investigated the effect of human recombinant IL-1 alone on SC expression in HT-29 cells in detail. Immunocytochemistry and Northern blot analysis revealed that IL-1 beta increased both the number of SC-positive cells and SC mRNA expression. Enzyme-linked immunosorbent assay revealed that IL-1 beta enhanced secretion by HT-29 cells in both time- and dose-dependent manners. IL-1 alpha had the same effects on HT-29 cells. Northern blot analysis demonstrated that cycloheximide and actinomycin D abolished the effect of IL-1. Moreover, we detected IL-1 receptor (IL-1R) type I mRNA in HT-29 cells by polymerase chain reaction (PCR) and sequenced the PCR-amplified product. We think that it reflects the possibility of the presence of IL-1R in HT-29 cells. From these data, we concluded that IL-1 beta and IL-1 alpha play regulatory roles in SC expression, and their effects depend on de novo protein synthesis and transcription.

Citing Articles

M1 macrophages evoke an increase in polymeric immunoglobulin receptor (PIGR) expression in MDA-MB468 breast cancer cells through secretion of interleukin-1β.

Asanprakit W, Lobo D, Eremin O, Bennett A Sci Rep. 2022; 12(1):16842.

PMID: 36207349 PMC: 9546936. DOI: 10.1038/s41598-022-20811-6.

Secretory Cells Are the Primary Source of pIgR in Small Airways.

Blackburn J, Schaff J, Gutor S, Du R, Nichols D, Sherrill T Am J Respir Cell Mol Biol. 2022; 67(3):334-345.

PMID: 35687143 PMC: 9447142. DOI: 10.1165/rcmb.2021-0548OC.

Prognostic significance of NY-ESO-1 antigen and PIGR expression in esophageal tumors of CHP-NY-ESO-1-vaccinated patients as adjuvant therapy.

Nagata Y, Kageyama S, Ishikawa T, Kokura S, Okayama T, Abe T Cancer Immunol Immunother. 2022; 71(11):2743-2755.

PMID: 35429246 PMC: 10992970. DOI: 10.1007/s00262-022-03194-5.

A Bioinformatic Analysis of Correlations between Polymeric Immunoglobulin Receptor (PIGR) and Liver Fibrosis Progression.

Zhang Y, Lu W, Chen X, Cao Y, Yang Z Biomed Res Int. 2021; 2021:5541780.

PMID: 33937393 PMC: 8055406. DOI: 10.1155/2021/5541780.

Role of Polymeric Immunoglobulin Receptor in IgA and IgM Transcytosis.

Wei H, Wang J Int J Mol Sci. 2021; 22(5).

PMID: 33668983 PMC: 7956327. DOI: 10.3390/ijms22052284.

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