Rapid Discrimination of Mycobacterium Tuberculosis Complex Strains by Ligation-mediated PCR Fingerprint Analysis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1997 Dec 17

PMID 9399550

Citations 23

Authors

G Prodhom

C Guilhot

M C Gutierrez

A Varnerot

B Gicquel

V Vincent

Affiliations

Soon will be listed here.

Abstract

A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.

Citing Articles

Automated IS6110-based fingerprinting of Mycobacterium tuberculosis: Reaching unprecedented discriminatory power and versatility.

Dekhil N, Skhairia M, Mhenni B, Fradj S, Warren R, Mardassi H PLoS One. 2018; 13(6):e0197913.

PMID: 29856789 PMC: 5983439. DOI: 10.1371/journal.pone.0197913.

PyroTyping, a novel pyrosequencing-based assay for Mycobacterium tuberculosis genotyping.

Molina-Moya B, Lacoma A, Garcia-Sierra N, Blanco S, Haba L, Samper S Sci Rep. 2017; 7(1):6777.

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Transposition mechanism, molecular characterization and evolution of IS6110, the specific evolutionary marker of Mycobacterium tuberculosis complex.

Thabet S, Souissi N Mol Biol Rep. 2016; 44(1):25-34.

PMID: 27766569 DOI: 10.1007/s11033-016-4084-x.

Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.

Jagielski T, Minias A, van Ingen J, Rastogi N, Brzostek A, Zaczek A Clin Microbiol Rev. 2016; 29(2):239-90.

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Comparison of ligation-mediated PCR methods in differentiation of Mycobacterium tuberculosis strains.

Zaczek A, Brzostek A, Wojtasik A, Sajduda A, Dziadek J Biomed Res Int. 2014; 2014:782071.

PMID: 24696162 PMC: 3947874. DOI: 10.1155/2014/782071.

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