Genotyping of Mycobacterium Tuberculosis with Additional Markers Enhances Accuracy in Epidemiological Studies

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1996 Sep 1

PMID 8862588

Citations 19

Authors

R Warren

M Richardson

S Sampson

J H Hauman

N Beyers

P R Donald

P D Van Helden

Affiliations

Soon will be listed here.

Abstract

Two highly polymorphic Mycobacterium tuberculosis genomic domains, characterized by hybridization to the oligonucleotide (GTG)5, were identified as potential DNA fingerprinting probes. These domains were cloned [pMTB484(1) and pMTB484(2K4), respectively] and shown to be useful for genotype analysis by Southern blotting. These probes were used to genotype geographically linked strains of M. tuberculosis previously shown to have identical IS6110 fingerprints. Subsequent DNA fingerprints generated with MTB484(1) and MTB484(2K4) showed a high degree of polymorphism, allowing subclassification of IS6110-defined clusters into composites of smaller clusters and unique strains. Correlation of the molecular data with patient interviews and clinical records confirmed the sensitivity of these probes, as contacts were established only within subclusters. These findings demonstrate the requirement for multiple probes to accurately classify M. tuberculosis strains, even those with high copy numbers of IS6110. The enhanced accuracy of strain typing should, in turn, further our understanding of the epidemiology of tuberculosis.

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References

van Soolingen D, de Haas P, Hermans P, Groenen P, van Embden J . Comparison of various repetitive DNA elements as genetic markers for strain differentiation and epidemiology of Mycobacterium tuberculosis. J Clin Microbiol. 1993; 31(8):1987-95. PMC: 265684. DOI: 10.1128/jcm.31.8.1987-1995.1993. View

Yuen L, Ross B, Jackson K, Dwyer B . Characterization of Mycobacterium tuberculosis strains from Vietnamese patients by Southern blot hybridization. J Clin Microbiol. 1993; 31(6):1615-8. PMC: 265589. DOI: 10.1128/jcm.31.6.1615-1618.1993. View

Small P, Moss A . Molecular epidemiology and the new tuberculosis. Infect Agents Dis. 1993; 2(3):132-8. View

Small P, Hopewell P, Singh S, Paz A, Parsonnet J, Ruston D . The epidemiology of tuberculosis in San Francisco. A population-based study using conventional and molecular methods. N Engl J Med. 1994; 330(24):1703-9. DOI: 10.1056/NEJM199406163302402. View

Alland D, Kalkut G, Moss A, McAdam R, Hahn J, Bosworth W . Transmission of tuberculosis in New York City. An analysis by DNA fingerprinting and conventional epidemiologic methods. N Engl J Med. 1994; 330(24):1710-6. DOI: 10.1056/NEJM199406163302403. View

Valway S, Richards S, Kovacovich J, Greifinger R, Crawford J, DOOLEY S . Outbreak of multi-drug-resistant tuberculosis in a New York State prison, 1991. Am J Epidemiol. 1994; 140(2):113-22. DOI: 10.1093/oxfordjournals.aje.a117222. View

Wiid I, Werely C, Beyers N, Donald P, Van Helden P . Oligonucleotide (GTG)5 as a marker for Mycobacterium tuberculosis strain identification. J Clin Microbiol. 1994; 32(5):1318-21. PMC: 263681. DOI: 10.1128/jcm.32.5.1318-1321.1994. View

van Soolingen D, de Haas P, Hermans P, van Embden J . DNA fingerprinting of Mycobacterium tuberculosis. Methods Enzymol. 1994; 235:196-205. DOI: 10.1016/0076-6879(94)35141-4. View

Goyal M, Ormerod L, Shaw R . Epidemiology of an outbreak of drug-resistant tuberculosis in the U.K. using restriction fragment length polymorphism. Clin Sci (Lond). 1994; 86(6):749-51. DOI: 10.1042/cs0860749. View

10.

Hermans P, Messadi F, Guebrexabher H, van Soolingen D, de Haas P, Heersma H . Analysis of the population structure of Mycobacterium tuberculosis in Ethiopia, Tunisia, and The Netherlands: usefulness of DNA typing for global tuberculosis epidemiology. J Infect Dis. 1995; 171(6):1504-13. DOI: 10.1093/infdis/171.6.1504. View

11.

Torrea G, LeVee G, Grimont P, Martin C, Chanteau S, Gicquel B . Chromosomal DNA fingerprinting analysis using the insertion sequence IS6110 and the repetitive element DR as strain-specific markers for epidemiological study of tuberculosis in French Polynesia. J Clin Microbiol. 1995; 33(7):1899-904. PMC: 228294. DOI: 10.1128/jcm.33.7.1899-1904.1995. View

12.

Beyers N, Gie R, Zietsman H, Kunneke M, Hauman J, Tatley M . The use of a geographical information system (GIS) to evaluate the distribution of tuberculosis in a high-incidence community. S Afr Med J. 1996; 86(1):40-1, 44. View

13.

Warren R, Hauman J, Beyers N, Richardson M, Schaaf H, Donald P . Unexpectedly high strain diversity of Mycobacterium tuberculosis in a high-incidence community. S Afr Med J. 1996; 86(1):45-9. View

14.

Hermans P, van Soolingen D, Bik E, de Haas P, Dale J, van Embden J . Insertion element IS987 from Mycobacterium bovis BCG is located in a hot-spot integration region for insertion elements in Mycobacterium tuberculosis complex strains. Infect Immun. 1991; 59(8):2695-705. PMC: 258075. DOI: 10.1128/iai.59.8.2695-2705.1991. View

15.

Collins D, Stephens D . Identification of an insertion sequence, IS1081, in Mycobacterium bovis. FEMS Microbiol Lett. 1991; 67(1):11-5. DOI: 10.1016/0378-1097(91)90435-d. View

16.

Ross B, Raios K, Jackson K, Dwyer B . Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool. J Clin Microbiol. 1992; 30(4):942-6. PMC: 265190. DOI: 10.1128/jcm.30.4.942-946.1992. View

17.

Hermans P, van Soolingen D, van Embden J . Characterization of a major polymorphic tandem repeat in Mycobacterium tuberculosis and its potential use in the epidemiology of Mycobacterium kansasii and Mycobacterium gordonae. J Bacteriol. 1992; 174(12):4157-65. PMC: 206128. DOI: 10.1128/jb.174.12.4157-4165.1992. View

18.

van Soolingen D, Hermans P, de Haas P, van Embden J . Insertion element IS1081-associated restriction fragment length polymorphisms in Mycobacterium tuberculosis complex species: a reliable tool for recognizing Mycobacterium bovis BCG. J Clin Microbiol. 1992; 30(7):1772-7. PMC: 265379. DOI: 10.1128/jcm.30.7.1772-1777.1992. View

19.

Godfrey-Faussett P, Mortimer P, Jenkins P, Stoker N . Evidence of transmission of tuberculosis by DNA fingerprinting. BMJ. 1992; 305(6847):221-3. PMC: 1882652. DOI: 10.1136/bmj.305.6847.221. View

20.

van Embden J, Cave M, Crawford J, Dale J, Eisenach K, Gicquel B . Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993; 31(2):406-9. PMC: 262774. DOI: 10.1128/jcm.31.2.406-409.1993. View