Development of a Novel Antigen Detection Test for Histoplasmosis

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1997 Oct 8

PMID 9316918

Citations 32

Authors

B L Gomez

J I Figueroa

A J Hamilton

B L Ortiz

M A Robledo

A Restrepo

R J Hay

Affiliations

Soon will be listed here.

Abstract

Histoplasmosis is an important systemic fungal infection, particularly among immunocompromised individuals living or travelling in areas of endemicity, who, without antifungal therapy, may develop a progressive disseminated fatal infection. For such patients, the detection of antibody responses by immunodiffusion or complement fixation test is of limited use. In contrast, the detection of Histoplasma capsulatum circulating antigens may provide a more practical approach to the rapid diagnosis of the disease. Accordingly, an inhibition enzyme-linked immunosorbent assay (ELISA) for the detection of a 69- to 70-kDa H. capsulatum-specific determinant and incorporating a species-specific murine monoclonal antibody was developed. With sera from patients with different forms of the disease (n = 35), the overall sensitivity of the test was found to be 71.4%, while the specificity was found to be 98% with normal human sera from areas of endemicity (n = 44) and 85.4% with sera from patients with other chronic fungal or bacterial infections (n = 48). This novel, highly specific ELISA provides a significant addition to the existing diagnostic tests for the detection of histoplasmosis.

Citing Articles

Histoplasma capsulatum urinary antigen detection in a kidney transplant recipient with acute paracoccidioidomycosis: Case study and literature review.

Bastos M, Varon A, Theodoro P, Falcao E, Zancope-Oliveira R, do Valle A PLoS Negl Trop Dis. 2024; 18(8):e0012472.

PMID: 39208382 PMC: 11389928. DOI: 10.1371/journal.pntd.0012472.

The Current and Future States of Diagnostic Tests for Histoplasmosis with a Focus on People with HIV and Disseminated Histoplasmosis.

Villareal K, Price A, Pasqualotto A, Bahr N J Fungi (Basel). 2023; 9(8).

PMID: 37623564 PMC: 10456117. DOI: 10.3390/jof9080793.

Immunologic Diagnosis of Endemic Mycoses.

Almeida-Paes R, Bernardes-Engemann A, Motta B, Pizzini C, Almeida M, Muniz M J Fungi (Basel). 2022; 8(10).

PMID: 36294558 PMC: 9605100. DOI: 10.3390/jof8100993.

Closing gaps in histoplasmosis: clinical characteristics and factors associated with probable/histoplasmosis in HIV/AIDS hospitalized patients, a retrospective cross-sectional study in two tertiary centers in Pereira, Colombia.

Hoyos Pulgarin J, Alzate Piedrahita J, Moreno Gomez G, Sierra Palacio J, Ordonez K, Arias Ramos D AIDS Res Ther. 2021; 18(1):51.

PMID: 34384448 PMC: 8358261. DOI: 10.1186/s12981-021-00377-5.

Difficult to Diagnose: An Unusual Cause of Cavitary Lung Lesion.

Norman H, Davis J, Kadaria D Am J Case Rep. 2020; 21:e921274.

PMID: 32449900 PMC: 7274497. DOI: 10.12659/AJCR.921274.

References

Wheat L, Baker R, Curfman M, Eads M, Israel K, Norris S . Disseminated histoplasmosis in the acquired immune deficiency syndrome: clinical findings, diagnosis and treatment, and review of the literature. Medicine (Baltimore). 1990; 69(6):361-74. DOI: 10.1097/00005792-199011000-00004. View

Figueroa J, Hamilton A, Bartholomew M, Harada T, Fenelon L, Hay R . Preparation of species-specific murine monoclonal antibodies against the yeast phase of Paracoccidioides brasiliensis. J Clin Microbiol. 1990; 28(8):1766-9. PMC: 268044. DOI: 10.1128/jcm.28.8.1766-1769.1990. View

Roque-Barreira M . Antigenemia in paracoccidioidomycosis. J Clin Microbiol. 1992; 30(2):381-5. PMC: 265064. DOI: 10.1128/jcm.30.2.381-385.1992. View

Wheat L, Williams B, Connolly K, Blair R, Bartlett M, Durkin M . Diagnosis of histoplasmosis in patients with the acquired immunodeficiency syndrome by detection of Histoplasma capsulatum polysaccharide antigen in bronchoalveolar lavage fluid. Am Rev Respir Dis. 1992; 145(6):1421-4. DOI: 10.1164/ajrccm/145.6.1421. View

Torres M, Diaz H, Herrera T, Sada E . Evaluation of enzyme linked immunosorbent-assay and western blot for diagnosis of histoplasmosis. Rev Invest Clin. 1993; 45(2):155-60. View

MORENO A, Isturiz R . Fungal infection in Latin American countries. Infect Dis Clin North Am. 1994; 8(1):129-54. View

Williams B, Fojtasek M, Wheat J . Diagnosis of histoplasmosis by antigen detection during an outbreak in Indianapolis, Ind. Arch Pathol Lab Med. 1994; 118(12):1205-8. View

Fojtasek M, Kleiman M, Blair R, Wheat L . The Histoplasma capsulatum antigen assay in disseminated histoplasmosis in children. Pediatr Infect Dis J. 1994; 13(9):801-5. DOI: 10.1097/00006454-199409000-00010. View

Kaufman L, Standard P, Anderson S, Jalbert M, Swisher B . Development of specific fluorescent-antibody test for tissue form of Penicillium marneffei. J Clin Microbiol. 1995; 33(8):2136-8. PMC: 228349. DOI: 10.1128/jcm.33.8.2136-2138.1995. View

10.

Garner J, Kernodle D . False-positive Histoplasma antigen test in a patient with pulmonary blastomycosis. Clin Infect Dis. 1995; 21(4):1054. DOI: 10.1093/clinids/21.4.1054. View

11.

Bradsher R . Histoplasmosis and blastomycosis. Clin Infect Dis. 1996; 22 Suppl 2:S102-11. DOI: 10.1093/clinids/22.supplement_2.s102. View

12.

Bava A . Histoplasmosis in the Muñiz Hospital of Buenos Aires. Rev Inst Med Trop Sao Paulo. 1995; 37(6):531-5. DOI: 10.1590/s0036-46651995000600010. View

13.

Tobon A, Franco L, Espinal D, Gomez I, Arango M, Trujillo H . Disseminated histoplasmosis in children: the role of itraconazole therapy. Pediatr Infect Dis J. 1996; 15(11):1002-8. DOI: 10.1097/00006454-199611000-00014. View

14.

Wheat J . Histoplasmosis in the acquired immunodeficiency syndrome. Curr Top Med Mycol. 1996; 7(1):7-18. View

15.

Wheat L, Blair R, Connolly K, Garringer T, Katz B . Histoplasmosis relapse in patients with AIDS: detection using Histoplasma capsulatum variety capsulatum antigen levels. Ann Intern Med. 1991; 115(12):936-41. DOI: 10.7326/0003-4819-115-12-936. View

16.

GOODWIN Jr R, SHAPIRO J, Thurman G, Thurman S, DES PREZ R . Disseminated histoplasmosis: clinical and pathologic correlations. Medicine (Baltimore). 1980; 59(1):1-33. View

17.

Wheat L, Slama T, Eitzen H, Kohler R, French M, Biesecker J . A large urban outbreak of histoplasmosis: clinical features. Ann Intern Med. 1981; 94(3):331-7. DOI: 10.7326/0003-4819-94-3-331. View

18.

Goodwin R, Loyd J, DES PREZ R . Histoplasmosis in normal hosts. Medicine (Baltimore). 1981; 60(4):231-66. DOI: 10.1097/00005792-198107000-00001. View

19.

Wheat L, Slama T, Norton J, Kohler R, Eitzen H, French M . Risk factors for disseminated or fatal histoplasmosis. Analysis of a large urban outbreak. Ann Intern Med. 1982; 96(2):159-63. DOI: 10.7326/0003-4819-96-2-159. View

20.

Wheat L, Kohler R, Tewari R . Diagnosis of disseminated histoplasmosis by detection of Histoplasma capsulatum antigen in serum and urine specimens. N Engl J Med. 1986; 314(2):83-8. DOI: 10.1056/NEJM198601093140205. View