Overexpression of an Endogenous Thionin Enhances Resistance of Arabidopsis Against Fusarium Oxysporum

Overview

Journal Plant Cell

Publisher Oxford University Press

Specialties Biology
Cell Biology

Date 1997 Apr 1

PMID 9144959

Citations 84

Authors

P Epple

K Apel

H Bohlmann

Affiliations

Soon will be listed here.

Abstract

Thionins are antimicrobial proteins that are thought to be involved in plant defense. Concordant with this view, we have recently shown that the Arabidopsis thionin Thi2.1 gene is inducible by phytopathogenic fungi. Here, we demonstrate that constitutive overexpression of this thionin enhances the resistance of the susceptible ecotype Columbia (Col-2) against attack by Fusarium oxysporum f sp matthiolae. Transgenic lines had a reduced loss of chlorophyll after inoculation and supported significantly less fungal growth on the cotyledons, as evaluated by trypan blue staining. Moreover, fungi on cotyledons of transgenic lines had more hyphae with growth anomalies, including hyperbranching, than on cotyledons of the parental line. No transcripts for pathogenesis-related PR-1, PR-5, or the pathogen-inducible plant defensin Pdf1.2 could be detected in uninoculated transgenic seedlings, indicating that all of the observed effects of the overexpressing lines are most likely the result of the toxicity of the THI2.1 thionin. Our findings strongly support the view that thionins are defense proteins.

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References

Alexander D, Goodman R, Gut-Rella M, Glascock C, Weymann K, Friedrich L . Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a. Proc Natl Acad Sci U S A. 1993; 90(15):7327-31. PMC: 47130. DOI: 10.1073/pnas.90.15.7327. View

Bohl S, Apel K . A novel fingerprint method for analyzing the expression of complex multigene families of very low transcript abundance. Plant J. 1993; 3(6):887-93. DOI: 10.1111/j.1365-313x.1993.00887.x. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Mauch-Mani B, Slusarenko A . Production of Salicylic Acid Precursors Is a Major Function of Phenylalanine Ammonia-Lyase in the Resistance of Arabidopsis to Peronospora parasitica. Plant Cell. 1996; 8(2):203-212. PMC: 161092. DOI: 10.1105/tpc.8.2.203. View

Herbers K, Meuwly P, Frommer W, Metraux J, Sonnewald U . Systemic Acquired Resistance Mediated by the Ectopic Expression of Invertase: Possible Hexose Sensing in the Secretory Pathway. Plant Cell. 1996; 8(5):793-803. PMC: 161138. DOI: 10.1105/tpc.8.5.793. View

Epple P, Apel K, Bohlmann H . An Arabidopsis thaliana thionin gene is inducible via a signal transduction pathway different from that for pathogenesis-related proteins. Plant Physiol. 1995; 109(3):813-20. PMC: 161381. DOI: 10.1104/pp.109.3.813. View

Mittler R, Shulaev V, Lam E . Coordinated Activation of Programmed Cell Death and Defense Mechanisms in Transgenic Tobacco Plants Expressing a Bacterial Proton Pump. Plant Cell. 1995; 7(1):29-42. PMC: 160762. DOI: 10.1105/tpc.7.1.29. View

Mittler R, Lam E . Sacrifice in the face of foes: pathogen-induced programmed cell death in plants. Trends Microbiol. 1996; 4(1):10-15. DOI: 10.1016/0966-842x(96)81499-5. View

Melzer S, Majewski D, Apel K . Early Changes in Gene Expression during the Transition from Vegetative to Generative Growth in the Long-Day Plant Sinapis alba. Plant Cell. 1990; 2(10):953-961. PMC: 159944. DOI: 10.1105/tpc.2.10.953. View

10.

Yang H, McLeese J, Weisbart M, Dionne J, Lemaire I, Aubin R . Simplified high throughput protocol for northern hybridization. Nucleic Acids Res. 1993; 21(14):3337-8. PMC: 309787. DOI: 10.1093/nar/21.14.3337. View

11.

Terras F, Eggermont K, Kovaleva V, Raikhel N, OSBORN R, Kester A . Small cysteine-rich antifungal proteins from radish: their role in host defense. Plant Cell. 1995; 7(5):573-88. PMC: 160805. DOI: 10.1105/tpc.7.5.573. View

12.

Florack D, Dirkse W, Visser B, Heidekamp F, Stiekema W . Expression of biologically active hordothionins in tobacco. Effects of pre- and pro-sequences at the amino and carboxyl termini of the hordothionin precursor on mature protein expression and sorting. Plant Mol Biol. 1994; 24(1):83-96. DOI: 10.1007/BF00040576. View

13.

Schagger H, von Jagow G . Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem. 1987; 166(2):368-79. DOI: 10.1016/0003-2697(87)90587-2. View

14.

Thevissen K, Ghazi A, De Samblanx G, Brownlee C, OSBORN R, Broekaert W . Fungal membrane responses induced by plant defensins and thionins. J Biol Chem. 1996; 271(25):15018-25. DOI: 10.1074/jbc.271.25.15018. View

15.

Holsters M, De Waele D, Depicker A, Messens E, Van Montagu M, Schell J . Transfection and transformation of Agrobacterium tumefaciens. Mol Gen Genet. 1978; 163(2):181-7. DOI: 10.1007/BF00267408. View

16.

Garcia-Olmedo F, Molina A, Segura A, Moreno M . The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol. 1995; 3(2):72-4. DOI: 10.1016/s0966-842x(00)88879-4. View

17.

Feinberg A, Vogelstein B . A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem. 1983; 132(1):6-13. DOI: 10.1016/0003-2697(83)90418-9. View

18.

Terras F, Schoofs H, Thevissen K, OSBORN R, Vanderleyden J, Cammue B . Synergistic Enhancement of the Antifungal Activity of Wheat and Barley Thionins by Radish and Oilseed Rape 2S Albumins and by Barley Trypsin Inhibitors. Plant Physiol. 1993; 103(4):1311-1319. PMC: 159121. DOI: 10.1104/pp.103.4.1311. View

19.

Bevan M . Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res. 1984; 12(22):8711-21. PMC: 320409. DOI: 10.1093/nar/12.22.8711. View

20.

Gaffney T, Friedrich L, Vernooij B, Negrotto D, Nye G, Uknes S . Requirement of salicylic Acid for the induction of systemic acquired resistance. Science. 1993; 261(5122):754-6. DOI: 10.1126/science.261.5122.754. View