Transfection and Transformation of Agrobacterium Tumefaciens

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1978 Jul 11

PMID 355847

Citations 384

Authors

M Holsters

D De Waele

A Depicker

E Messens

M Van Montagu

J Schell

Affiliations

Soon will be listed here.

Abstract

The freeze thaw transfection procedure of Dityatkin et al. (1972) was adapted for the transfection and transformation of A. tumefaciens. Transfection of the strains B6S3 and B6-6 with DNA of the temperate phage PS8cc186 yielded a maximum frequency of 2 10(-7) transfectants per total recipient population. In transformation of the strain GV3100 with the P type plasmid RP4 a maximum frequency of 3.5 10(-7) transformants per total recipient population was obtained. Agrobacterium Ti-plasmids were introduced in the strain GV3100 with a maximal efficiency of 4.5 10(-8). These experiments provide further evidence that the Ti-plasmid is responsible for the oncogenic properties of A tumefaciens and for its capacity to induce "opine" synthesis in Crown-gall plant cells.

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