Inhibition of RecA-mediated Cleavage in Covalent Dimers of UmuD

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1996 Dec 1

PMID 8955416

Citations 5

Authors

M H Lee

A Guzzo

G C Walker

Affiliations

Soon will be listed here.

Abstract

Disulfide-cross-linked UmuD2 derivatives were cleaved poorly upon incubation with activated RecA. Reducing the disulfide bonds prior to incubating the derivatives with RecA dramatically increased their extent of cleavage. These observations suggest that the UmuD monomer is a better substrate for the RecA-mediated cleavage reaction than the dimer.

Citing Articles

The dimeric SOS mutagenesis protein UmuD is active as a monomer.

Ollivierre J, Sikora J, Beuning P J Biol Chem. 2010; 286(5):3607-17.

PMID: 21118802 PMC: 3030365. DOI: 10.1074/jbc.M110.167254.

Regulation of Escherichia coli SOS mutagenesis by dimeric intrinsically disordered umuD gene products.

Simon S, Sousa F, Mohana-Borges R, Walker G Proc Natl Acad Sci U S A. 2008; 105(4):1152-7.

PMID: 18216271 PMC: 2234107. DOI: 10.1073/pnas.0706067105.

A role for the umuDC gene products of Escherichia coli in increasing resistance to DNA damage in stationary phase by inhibiting the transition to exponential growth.

Murli S, Opperman T, Smith B, Walker G J Bacteriol. 2000; 182(4):1127-35.

PMID: 10648540 PMC: 94390. DOI: 10.1128/JB.182.4.1127-1135.2000.

A model for a umuDC-dependent prokaryotic DNA damage checkpoint.

Opperman T, Murli S, Smith B, Walker G Proc Natl Acad Sci U S A. 1999; 96(16):9218-23.

PMID: 10430923 PMC: 17760. DOI: 10.1073/pnas.96.16.9218.

Interactions of Escherichia coli UmuD with activated RecA analyzed by cross-linking UmuD monocysteine derivatives.

Lee M, Walker G J Bacteriol. 1996; 178(24):7285-94.

PMID: 8955414 PMC: 178645. DOI: 10.1128/jb.178.24.7285-7294.1996.

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