The Influence of Cytokines on the Integrity of the Blood-brain Barrier in Vitro

Overview

Journal J Neuroimmunol

Specialty Neurology

Date 1996 Jan 1

PMID 8598388

Citations 222

Authors

H E de Vries

M van Oosten

A G De Boer

T J Van Berkel

D D Breimer

J Kuiper

Affiliations

Soon will be listed here.

Abstract

The effects of the cytokines tumour necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta and IL-6 on the permeability of monolayers of rat cerebral endothelial cells (RCEC) were investigated to assess potential changes in the integrity of the blood-brain barrier (BBB). RCEC were cultured to tight monolayers with a trans endothelial electrical resistance (TEER) of 100-150 ohm . cm2 on polycarbonate filters. Exposure of the RCEC to TNF-alpha, IL-1 beta and IL-6 induced a decline in the TEER, which could be completely abolished by 1 muM of indomethacin, a cyclooxygenase inhibitor. In addition, the effect of IL-1 beta on TEER across monolayers of RCEC could be completely inhibited by IL-1 receptor antagonist. In conclusion, cytokines induce a disruption of the BBB in vitro. In this process, cyclooxygenase activation within the endothelial cells seems to play a key role.

Citing Articles

Cell signaling communication within papillary craniopharyngioma revealed by an integrated analysis of single-cell RNA sequencing and bulk RNA sequencing.

Zhu X, Jia Y, Zhao Z, Zhang X, Zhao Y, Gui S J Transl Med. 2025; 23(1):124.

PMID: 39871369 PMC: 11773883. DOI: 10.1186/s12967-025-06149-3.

The Role of Corticosteroids in Non-Bacterial and Secondary Encephalitis.

Di Flumeri G, Giaccari L, Pace M, Passavanti M, Pota V, Riccardi V Life (Basel). 2025; 14(12.

PMID: 39768405 PMC: 11679550. DOI: 10.3390/life14121699.

Value of systemic inflammation markers for the detection of minimal and prediction of overt hepatic encephalopathy after TIPS insertion.

Tiede A, Stockhoff L, Ehrenbauer A, Rieland H, Cornberg M, Meyer B Metab Brain Dis. 2024; 40(1):58.

PMID: 39656322 PMC: 11632008. DOI: 10.1007/s11011-024-01436-2.

Extracellular vesicles released by ALL patients contain HNE-adducted proteins: Implications of collateral damage.

Ho J, Sukati S, Taylor T, Carter S, Fuller B, Marmo A Free Radic Biol Med. 2024; 227:312-321.

PMID: 39643137 PMC: 11786608. DOI: 10.1016/j.freeradbiomed.2024.12.006.

Eleutheroside B alleviates oxidative stress and neuroinflammation by inhibiting the JAK2/STAT3 signaling pathway in a rat high altitude cerebral edema model.

He Y, Zhang H, Zhang X, Han Y, Duan H, Song W Front Pharmacol. 2024; 15:1506483.

PMID: 39619615 PMC: 11604411. DOI: 10.3389/fphar.2024.1506483.