Recombinant Human Interferon-inducible Protein 10 is a Chemoattractant for Human Monocytes and T Lymphocytes and Promotes T Cell Adhesion to Endothelial Cells

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1993 Jun 1

PMID 8496693

Citations 249

Authors

D D Taub

A R Lloyd

K Conlon

J M Wang

J R Ortaldo

A Harada

K Matsushima

D J Kelvin

J J Oppenheim

Affiliations

Soon will be listed here.

Abstract

The human cytokine interferon-inducible protein 10 (IP-10) is a small glycoprotein secreted by activated T cells, monocytes, endothelial cells, and keratinocytes, and is structurally related to a family of chemotactic cytokines called chemokines. Although this protein is present in sites of delayed-type hypersensitivity reactions and lepromatous leprosy lesions, the biological activity of IP-10 remains unknown. We report here that recombinant human IP-10 stimulated significant in vitro chemotaxis of human peripheral blood monocytes but not neutrophils. Recombinant human IP-10 also stimulated chemotaxis of stimulated, but not unstimulated, human peripheral blood T lymphocytes. Phenotypic analysis of the stimulated T cell population responsive to IP-10 demonstrated that stimulated CD4+ and CD29+ T cells migrated in response to IP-10. This resembles the biological activity of the previously described T cell chemoattractant RANTES. Using an endothelial cell adhesion assay, we demonstrated that stimulated T cells pretreated with optimal doses of IP-10 exhibited a greatly enhanced ability to bind to an interleukin 1-treated endothelial cell monolayer. These results demonstrate that the IP-10 gene encodes for an inflammatory mediator that specifically stimulates the directional migration of T cells and monocytes as well as potentiates T cell adhesion to endothelium.

Citing Articles

Role of CXCL10 released from osteocytes in response to TNF-α stimulation on osteoclasts.

Miura M, Kitaura H, Ohori F, Narita K, Ren J, Noguchi T Sci Rep. 2025; 15(1):3040.

PMID: 39856227 PMC: 11760356. DOI: 10.1038/s41598-025-87092-7.

The Microenvironment in DCIS and Its Role in Disease Progression.

Roozitalab M, Prekete N, Allen M, Grose R, Jones J Adv Exp Med Biol. 2025; 1464():211-235.

PMID: 39821028 DOI: 10.1007/978-3-031-70875-6_12.

Study on the changes and significance of CXCL10 level in serum of isolated polymyalgia rheumatica.

Shuai X, Dandan F, Liang X Clin Rheumatol. 2024; 43(12):3993-3998.

PMID: 39476056 PMC: 11582166. DOI: 10.1007/s10067-024-07209-7.

Urinary Chemokines CXCL9 and CXCL10 Are Non-Invasive Biomarkers of Kidney Transplant Rejection.

Macioniene E, Simanavicius M, Vitkauskaite M, Vickiene A, Stauce R, Vinikovas A Ann Transplant. 2024; 29:e944762.

PMID: 39402819 PMC: 11490196. DOI: 10.12659/AOT.944762.

Serum Concentrations of Chemokines CCL20, CXCL8 and CXCL10 in Relapsing-Remitting Multiple Sclerosis and Their Association with Presence of Antibodies against Epstein-Barr Virus.

Koscak Lukac J, Baronica K, Sucur A, Sremec J, Tomasovic S, Baronica R Int J Mol Sci. 2024; 25(15).

PMID: 39125633 PMC: 11311471. DOI: 10.3390/ijms25158064.

References

Jaffe E, Nachman R, Becker C, Minick C . Culture of human endothelial cells derived from umbilical veins. Identification by morphologic and immunologic criteria. J Clin Invest. 1973; 52(11):2745-56. PMC: 302542. DOI: 10.1172/JCI107470. View

Pardi R, Inverardi L, Bender J . Regulatory mechanisms in leukocyte adhesion: flexible receptors for sophisticated travelers. Immunol Today. 1992; 13(6):224-30. DOI: 10.1016/0167-5699(92)90159-5. View

Luster A, Unkeless J, Ravetch J . Gamma-interferon transcriptionally regulates an early-response gene containing homology to platelet proteins. Nature. 1985; 315(6021):672-6. DOI: 10.1038/315672a0. View

Luster A, Ravetch J . Biochemical characterization of a gamma interferon-inducible cytokine (IP-10). J Exp Med. 1987; 166(4):1084-97. PMC: 2188708. DOI: 10.1084/jem.166.4.1084. View

Kaplan G, Luster A, Hancock G, COHN Z . The expression of a gamma interferon-induced protein (IP-10) in delayed immune responses in human skin. J Exp Med. 1987; 166(4):1098-108. PMC: 2188712. DOI: 10.1084/jem.166.4.1098. View

Luster A, Ravetch J . Genomic characterization of a gamma-interferon-inducible gene (IP-10) and identification of an interferon-inducible hypersensitive site. Mol Cell Biol. 1987; 7(10):3723-31. PMC: 368028. DOI: 10.1128/mcb.7.10.3723-3731.1987. View

June C, Ledbetter J, Gillespie M, LINDSTEN T, Thompson C . T-cell proliferation involving the CD28 pathway is associated with cyclosporine-resistant interleukin 2 gene expression. Mol Cell Biol. 1987; 7(12):4472-81. PMC: 368131. DOI: 10.1128/mcb.7.12.4472-4481.1987. View

Larsen C, Anderson A, Appella E, Oppenheim J, Matsushima K . The neutrophil-activating protein (NAP-1) is also chemotactic for T lymphocytes. Science. 1989; 243(4897):1464-6. DOI: 10.1126/science.2648569. View

Schall T, Bacon K, Toy K, Goeddel D . Selective attraction of monocytes and T lymphocytes of the memory phenotype by cytokine RANTES. Nature. 1990; 347(6294):669-71. DOI: 10.1038/347669a0. View

10.

Pilaro A, Sayers T, McCORMICK K, Reynolds C, Wiltrout R . An improved in vitro assay to quantitate chemotaxis of rat peripheral blood large granular lymphocytes (LGL). J Immunol Methods. 1990; 135(1-2):213-23. DOI: 10.1016/0022-1759(90)90275-z. View

11.

Shimizu Y, Shaw S, Graber N, Gopal T, Horgan K, van Seventer G . Activation-independent binding of human memory T cells to adhesion molecule ELAM-1. Nature. 1991; 349(6312):799-802. DOI: 10.1038/349799a0. View

12.

Schall T . Biology of the RANTES/SIS cytokine family. Cytokine. 1991; 3(3):165-83. DOI: 10.1016/1043-4666(91)90013-4. View

13.

Oppenheim J, Zachariae C, Mukaida N, Matsushima K . Properties of the novel proinflammatory supergene "intercrine" cytokine family. Annu Rev Immunol. 1991; 9:617-48. DOI: 10.1146/annurev.iy.09.040191.003153. View

14.

Enk A, Katz S . Early molecular events in the induction phase of contact sensitivity. Proc Natl Acad Sci U S A. 1992; 89(4):1398-402. PMC: 48458. DOI: 10.1073/pnas.89.4.1398. View

15.

Miller M, Krangel M . The human cytokine I-309 is a monocyte chemoattractant. Proc Natl Acad Sci U S A. 1992; 89(7):2950-4. PMC: 48781. DOI: 10.1073/pnas.89.7.2950. View

16.

Shimizu Y, Newman W, Tanaka Y, Shaw S . Lymphocyte interactions with endothelial cells. Immunol Today. 1992; 13(3):106-12. DOI: 10.1016/0167-5699(92)90151-V. View

17.

Falk W, Goodwin Jr R, Leonard E . A 48-well micro chemotaxis assembly for rapid and accurate measurement of leukocyte migration. J Immunol Methods. 1980; 33(3):239-47. DOI: 10.1016/0022-1759(80)90211-2. View