Quantitative Sequence-activity Models (QSAM)--tools for Sequence Design

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1993 Feb 11

PMID 8441682

Citations 18

Authors

J Jonsson

T Norberg

L Carlsson

C Gustafsson

S Wold

Affiliations

Soon will be listed here.

Abstract

Models have been developed that allow the biological activity of a DNA segment to be altered in a desired direction. Partial least squares projections to latent structures (PLS) was used to establish a quantitative model between a numerical description of 68 bp fragments of 25 E.coli promoters and their corresponding quantitative measure of in vivo strength. This quantitative sequence-activity model (QSAM) was used to generate two 68 bp fragments predicted to be more potent promoters than any of those on which the model originally was based. The optimized structures were experimentally verified to be strong promoters in vivo.

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