Synthesis of a Model Promoter for Gene Expression in Escherichia Coli

Overview

Journal Nucleic Acids Symp Ser

Publisher Oxford University Press

Specialties Biochemistry
Biotechnology

Date 1980 Jan 1

PMID 6265875

Citations 11

Authors

V N Dobrynin

V G Korobko

I V Severtsova

N S Bystrov

S A Chuvpilo

M N KOLOSOV

Affiliations

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Abstract

We have synthesized a 67-membered double-stranded DNA containing putative initiation sites for transcription and translation in E. coli. Among the potential applications of this DNA are construction of artificial genes, cloning of foreign genes in E. coli, and study of structure - activity relations in promoter and ribosome binding sites.

Citing Articles

Quantitative sequence-activity models (QSAM)--tools for sequence design.

Jonsson J, Norberg T, Carlsson L, Gustafsson C, Wold S Nucleic Acids Res. 1993; 21(3):733-9.

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Gene expression: chemical synthesis and molecular cloning of a bacteriophage T5 (T5P25) early promoter.

Rommens J, MacKnight D, Jay E Nucleic Acids Res. 1983; 11(17):5921-40.

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Biological expression of an Escherichia coli consensus sequence promoter and some mutant derivatives.

Rossi J, Soberon X, Marumoto Y, McMahon J, Itakura K Proc Natl Acad Sci U S A. 1983; 80(11):3203-7.

PMID: 6304698 PMC: 394008. DOI: 10.1073/pnas.80.11.3203.

Chemical synthesis and biochemical reactivity of bacteriophage lambda PR promoter.

deHaseth P, Goldman R, Cech C, Caruthers M Nucleic Acids Res. 1983; 11(3):773-87.

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Mouse ubiquitous B2 repeat in polysomal and cytoplasmic poly(A)+RNAs: uniderectional orientation and 3'-end localization.

Ryskov A, Ivanov P, Kramerov D, Georgiev G Nucleic Acids Res. 1983; 11(18):6541-58.

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