The Effects of Mutations in the Ant Promoter of Phage P22 Depend on Context

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1988 Oct 1

PMID 3143618

Citations 59

Authors

D Grana

T Gardella

M M Susskind

Affiliations

Soon will be listed here.

Abstract

Recombination was used to construct 22 two- or three-way combinations of down- and up-mutations in Pant, a strong, near-consensus promoter of phage P22. The relative strengths of these promoters in vivo were assayed by fusing them to an ant/lacZ gene fusion and measuring beta-galactosidase levels produced by lysogens carrying the fusions on single-copy prophages. The results of these assays show that the magnitude of the effect of a promoter mutation can vary considerably when its context is changed by the presence of another mutation. In addition, as Pant approaches conformity with the consensus promoter sequence, the up-mutations decrease promoter strength, even though the same mutations increase promoter strength in the presence of a down-mutation. These context effects imply that individual consensus base pairs cannot be considered to contribute to promoter strength independently.

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