Effects of Hyaluronic Acid on Macrophage Phagocytosis and Active Oxygen Release

Overview

Journal Agents Actions

Specialty Pharmacology

Date 1993 Jan 1

PMID 8386900

Citations 22

Authors

Y Suzuki

T Yamaguchi

Affiliations

Soon will be listed here.

Abstract

The effects of hyaluronic acid (HA) on macrophage function, in terms of the phagocytosis of latex beads and superoxide anion and hydrogen peroxide release stimulated by phorbol myristate acetate (PMA), were studied in guinea-pig peritoneal macrophages. Phagocytosis was inhibited in a dose- and molecular-weight-dependent manner by HA. The addition of PMA to the culture, at a dose of more than 10 ng ml-1, caused an increase in the release of active oxygens. The release of active oxygens was inhibited by high molecular weight HA (MW 2.02 x 10(6), HA-202) in a dose-dependent manner. In cell-free systems, HA-202 had a negligible effect in scavenging these active oxygens. Of the three molecular sizes of HA (MW: 0.28 x 10(6), 0.98 x 10(6) and 2.02 x 10(6)), HA-202 most strongly inhibited the active oxygen release. These results indicate that high-molecular-weight HA acts directly on macrophages to inhibit phagocytosis and active oxygen formation, which, in turn, ameliorates the progression of chronic inflammation.

Citing Articles

Hyaluronic acid impacts hematological endpoints and spleen histological features in African catfish (Clarias gariepinus).

Hamed M, Kotob M, Abou Khalil N, Anwari E, Bayoumie El Gazzar W, Idriss S BMC Vet Res. 2024; 20(1):294.

PMID: 38970005 PMC: 11225171. DOI: 10.1186/s12917-024-04113-9.

Hyaluronic Acid: Known for Almost a Century, but Still in Vogue.

Lierova A, Kasparova J, Filipova A, cizkova J, Pekarova L, Korecka L Pharmaceutics. 2022; 14(4).

PMID: 35456670 PMC: 9029726. DOI: 10.3390/pharmaceutics14040838.

Overcoming barriers for intra-articular delivery of disease-modifying osteoarthritis drugs.

Gao J, Xia Z, Mary H, Joseph J, Luo J, Joshi N Trends Pharmacol Sci. 2022; 43(3):171-187.

PMID: 35086691 PMC: 8840969. DOI: 10.1016/j.tips.2021.12.004.

Emerging Nano- and Micro-Technologies Used in the Treatment of Type-1 Diabetes.

Primavera R, Kevadiya B, Swaminathan G, Wilson R, De Pascale A, Decuzzi P Nanomaterials (Basel). 2020; 10(4).

PMID: 32325974 PMC: 7221526. DOI: 10.3390/nano10040789.

Glycosaminoglycan-Inspired Biomaterials for the Development of Bioactive Hydrogel Networks.

Neves M, Araujo M, Moroni L, da Silva R, Barrias C Molecules. 2020; 25(4).

PMID: 32098281 PMC: 7070556. DOI: 10.3390/molecules25040978.

References

Underhill C . The interaction of hyaluronate with the cell surface: the hyaluronate receptor and the core protein. Ciba Found Symp. 1989; 143:87-99; discussion 100-6, 281-5. DOI: 10.1002/9780470513774.ch6. View

Saari H . Oxygen derived free radicals and synovial fluid hyaluronate. Ann Rheum Dis. 1991; 50(6):389-92. PMC: 1004444. DOI: 10.1136/ard.50.6.389. View

Schalkwijk J, van den Berg W, van de Putte L, Joosten L . Hydrogen peroxide suppresses the proteoglycan synthesis of intact articular cartilage. J Rheumatol. 1985; 12(2):205-10. View

JOHNSTON Jr R, Godzik C, COHN Z . Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J Exp Med. 1978; 148(1):115-27. PMC: 2184904. DOI: 10.1084/jem.148.1.115. View

JOHNSTON Jr R, Lehmeyer J, Guthrie L . Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G. J Exp Med. 1976; 143(6):1551-6. PMC: 2190206. DOI: 10.1084/jem.143.6.1551. View

Revell P, Mayston V, Lalor P, Mapp P . The synovial membrane in osteoarthritis: a histological study including the characterisation of the cellular infiltrate present in inflammatory osteoarthritis using monoclonal antibodies. Ann Rheum Dis. 1988; 47(4):300-7. PMC: 1003511. DOI: 10.1136/ard.47.4.300. View

Merry P, Winyard P, Morris C, Grootveld M, Blake D . Oxygen free radicals, inflammation, and synovitis: and synovitis: the current status. Ann Rheum Dis. 1989; 48(10):864-70. PMC: 1003897. DOI: 10.1136/ard.48.10.864. View

Forrester J, Balazs E . Inhibition of phagocytosis by high molecular weight hyaluronate. Immunology. 1980; 40(3):435-46. PMC: 1458049. View

Dahl L, Dahl I, Engstrom-Laurent A, Granath K . Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies. Ann Rheum Dis. 1985; 44(12):817-22. PMC: 1001790. DOI: 10.1136/ard.44.12.817. View

10.

Bates E, Lowther D, Handley C . Oxygen free-radicals mediate an inhibition of proteoglycan synthesis in cultured articular cartilage. Ann Rheum Dis. 1984; 43(3):462-9. PMC: 1001371. DOI: 10.1136/ard.43.3.462. View

11.

Baker M, Feigan J, Lowther D . The mechanism of chondrocyte hydrogen peroxide damage. Depletion of intracellular ATP due to suppression of glycolysis caused by oxidation of glyceraldehyde-3-phosphate dehydrogenase. J Rheumatol. 1989; 16(1):7-14. View

12.

Baker M, Green S, Lowther D . Changes in the viscosity of hyaluronic acid after exposure to a myeloperoxidase-derived oxidant. Arthritis Rheum. 1989; 32(4):461-7. DOI: 10.1002/anr.1780320416. View

13.

Bradford M . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976; 72:248-54. DOI: 10.1016/0003-2697(76)90527-3. View

14.

de la Harpe J, Nathan C . A semi-automated micro-assay for H2O2 release by human blood monocytes and mouse peritoneal macrophages. J Immunol Methods. 1985; 78(2):323-36. DOI: 10.1016/0022-1759(85)90089-4. View

15.

Bates E, Lowther D, Johnson C . Hyaluronic acid synthesis in articular cartilage: an inhibition by hydrogen peroxide. Biochem Biophys Res Commun. 1985; 132(2):714-20. DOI: 10.1016/0006-291x(85)91191-x. View

16.

Sasada M, Kubo A, Nishimura T, Kakita T, Moriguchi T, Yamamoto K . Candidacidal activity of monocyte-derived human macrophages: relationship between Candida killing and oxygen radical generation by human macrophages. J Leukoc Biol. 1987; 41(4):289-94. DOI: 10.1002/jlb.41.4.289. View

17.

Sato H, Takahashi T, Ide H, Fukushima T, Tabata M, Sekine F . Antioxidant activity of synovial fluid, hyaluronic acid, and two subcomponents of hyaluronic acid. Synovial fluid scavenging effect is enhanced in rheumatoid arthritis patients. Arthritis Rheum. 1988; 31(1):63-71. DOI: 10.1002/art.1780310110. View