Interaction of Intronic Boundaries is Required for the Second Splicing Step Efficiency of a Group II Intron

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1993 Dec 15

PMID 8262060

Citations 19

Authors

G Chanfreau

A Jacquier

Affiliations

Soon will be listed here.

Abstract

Group II and nuclear pre-mRNAs introns share a common splicing pathway involving a lariat intermediate, as well as some primary sequence similarities at the splice junctions. In this work, we analyze the role of the conserved nucleotides at the first and penultimate positions (G1 and A886) of a group II self-splicing intron. We show that the G1 nucleotide is essential for the efficiency of both the first and the second splicing steps, while substitutions at the penultimate nucleotide affect mostly the efficiency of the second step. A reciprocal suppression of the second splicing step defect can be observed in some double mutants. This result is best explained by a non-Watson-Crick interaction between the first and the penultimate nucleotides of the intron, which occurs after lariat formation. The finding that an interaction between intron boundaries is required for the second splicing step in both group II and nuclear pre-mRNA introns strengthens the idea that both systems employ similar mechanisms, albeit with differences in the details of the nucleotide interactions.

Citing Articles

U5 snRNA Interactions With Exons Ensure Splicing Precision.

Artemyeva-Isman O, Porter A Front Genet. 2021; 12:676971.

PMID: 34276781 PMC: 8283771. DOI: 10.3389/fgene.2021.676971.

DNA cleavage and reverse splicing of ribonucleoprotein particles reconstituted with linear RmInt1 RNA.

Molina-Sanchez M, Toro N RNA Biol. 2019; 16(7):930-939.

PMID: 30943851 PMC: 6546360. DOI: 10.1080/15476286.2019.1601379.

Evolution of group II introns.

Zimmerly S, Semper C Mob DNA. 2015; 6:7.

PMID: 25960782 PMC: 4424553. DOI: 10.1186/s13100-015-0037-5.

In vitro characterization of the splicing efficiency and fidelity of the RmInt1 group II intron as a means of controlling the dispersion of its host mobile element.

Chillon I, Molina-Sanchez M, Fedorova O, Garcia-Rodriguez F, Martinez-Abarca F, Toro N RNA. 2014; 20(12):2000-10.

PMID: 25336586 PMC: 4238363. DOI: 10.1261/rna.047407.114.

Crystal structure of a eukaryotic group II intron lariat.

Robart A, Chan R, Peters J, Rajashankar K, Toor N Nature. 2014; 514(7521):193-7.

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