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Evaluation of Antibiotic Efficacy Using Electron Microscopy: Morphological Effects of Guanylureido Cephalosporin, Chlorobenzoylureido Cephalosporin, BL-P1654, and Carbenicillin on Pseudomonas Aeruginosa

Overview
Journal Antimicrob Agents Chemother
Publisher American Society for Microbiology
Specialty Pharmacology
Date 1976 Feb 1
PMID 817659
Citations 5
Authors
L F Ellis
D K Herron
D A Preston
L K Simmons
R A Schlegel
Affiliations
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Abstract

The response of Pseudomonas aeruginosa to carbenicillin, BL-P1654, and two cephalosporins (112384 and 112883) was evaluated by minimal inhibitory concentration determinations, [(14)C]leucine uptake studies, morphological studies of colonial growth, and mouse intraperitoneal inoculations. Spheroplast formation and bacterial lysis were not the early response; instead, cell division was inhibited and long filaments were formed. Spheroplast formation and bacterial lysis were not observed in the first 7 h of incubation in minimal inhibitory concentrations of antibiotic.

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References
1.
Dilworth C, Franklin M, Bance G . Morphological and cytological effects of carbenicillin on Pseudomonas pseudomallei. Can J Microbiol. 1974; 20(9):1229-33. DOI: 10.1139/m74-189. View
2.
Presslitz J, Ray V . DD-carboxypeptidase and peptidoglycan transpeptidase from Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1975; 7(5):578-81. PMC: 429185. DOI: 10.1128/AAC.7.5.578. View
3.
Prior R, Warner J . Morphological alterations of Pseudomonas aeruginosa by ticarcillin: a scanning electron microscope study. Antimicrob Agents Chemother. 1974; 6(6):853-5. PMC: 444749. DOI: 10.1128/AAC.6.6.853. View
4.
Nordstrom K, Sykes R . Effects of sublethal concentrations of benzylpenicillin on Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974; 6(6):741-6. PMC: 444729. DOI: 10.1128/AAC.6.6.741. View
5.
Nordstrom K, Sykes R . Induction kinetics of beta-lactamase biosynthesis in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1974; 6(6):734-40. PMC: 444728. DOI: 10.1128/AAC.6.6.734. View