Ribosomal Proteins from Streptomycin-resistant and Dependent Mutants, and Revertants from Streptomycin-dependence to Independence in Bacillus Subtilis

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1975 Sep 8

PMID 810662

Citations 9

Authors

T Ito

H Kosugi

K Higo

S Osawa

Affiliations

Soon will be listed here.

Abstract

Streptomycin-resistant and dependent mutants were isolated from Bacillus subtilis ATCC 6633. Ribosomal proteins were analysed from six such mutants with chromatography on carboxymethyl cellulose or phosphocellulose columns. An altered specific 30s ribosomal protein, 30B, could be detected in all of these mutants. Streptomycin-independent revertants were isolated from a streptomycin-dependent strain. Some of them had an altered 30s ribosomal protein, 30A, and some others had an altered 30C protein. It was shown that from the data of partial N-terminal amino acid sequences together with amino acid compositions and mobilities on two-dimensional gel electrophoresis of these proteins that 30A, 30B, and 30C proteins were homologous with S5, S12 and S4 of E. coli 30s ribosomal proteins, respectively.

Citing Articles

Selection in Bacillus subtilis giving rise to strains with mutational alterations in a variety of ribosomal proteins.

Dabbs E Mol Gen Genet. 1982; 187(2):297-301.

PMID: 6817030 DOI: 10.1007/BF00331132.

Revertants of a streptomycin-resistant, oligosporogenous mutant of Bacillus subtilis.

Henkin T, Campbell K, Chambliss G Mol Gen Genet. 1982; 186(3):347-54.

PMID: 6811835 DOI: 10.1007/BF00729453.

Genetic analysis of a streptomycin-resistant oligosporogenous Bacillus subtilis mutant.

Henkin T, Chambliss G J Bacteriol. 1984; 157(1):202-10.

PMID: 6418717 PMC: 215153. DOI: 10.1128/jb.157.1.202-210.1984.

Alteration of ribosomal protein S5 from two spectinomycin-resistant mutants of Bacillus subtilis: deletion of one amino acid residue.

Higo K, Itoh T, Osawa S Mol Gen Genet. 1982; 185(2):205-6.

PMID: 6211592 DOI: 10.1007/BF00330787.

Gene locus of a 30s ribosomal protein S20 of Bacillus subtilis.

Osawa S Mol Gen Genet. 1976; 144(1):49-51.

PMID: 817126 DOI: 10.1007/BF00277303.

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