Direct Evidence of a Heterotrimeric Complex of Human Interleukin-4 with Its Receptors

Overview

Journal Protein Sci

Specialty Biochemistry

Date 1995 Mar 1

PMID 7795521

Citations 4

Authors

R C Hoffman

B J Castner

M Gerhart

M G Gibson

B D Rasmussen

C J March

J Weatherbee

M Tsang

A Gustchina

C Schalk-Hihi

Affiliations

Soon will be listed here.

Abstract

The mode of binding of interleukin-4 (IL-4) to its two known receptors, specific receptor IL-4R and a shared receptor gamma c, was investigated using gel filtration and gel electrophoresis. A ternary complex between IL-4 and the soluble domains of the two receptors was shown to exist in solution. The association constant between gamma c and the stable complex of IL-4/sIL-4R is in the millimolar range, making the ternary complex a feasible target for crystallization studies.

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