Translation of T7 RNA in Vitro Without Cleavage by RNase III

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1976 Jun 1

PMID 775130

Citations 5

Authors

Y Yamada

D Nakada

Affiliations

Soon will be listed here.

Abstract

T7 early mRNA's are generated from a high-molecular-weight precursor RNA by site-specific RNase III cleavage. When T7 DNA is transcribed in vitro by Escherichia coli RNA polymerase, the transcript is a large, single-piece RNA equivalent to the in vivo precursor RNA. The T7 RNA synthesized in vitro can be translated as a polycistronic messenger without cleavage by RNase III. All T7 early proteins are synthesized in an RNase III-free, protein-synthesizing system directed by the uncleaved T7 RNA.

Citing Articles

Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

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Attenuation and processing of RNA from the rplJL--rpoBC transcription unit of Escherichia coli.

Barry G, Squires C, Squires C Proc Natl Acad Sci U S A. 1980; 77(6):3331-5.

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Nucleolytic processing of ribonucleic acid transcripts in procaryotes.

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Decay rates of Escherichia coli trp messenger RNA molecules lacking the normal 5'-terminal sequences.

Kano Y, Nakamura H, SOMERVILLE R, Imamoto F Mol Gen Genet. 1979; 176(3):379-84.

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