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Chemical Stability of Bacteriophage T7 Early MRNA

Overview
Journal J Virol
Publisher American Society for Microbiology
Date 1975 Dec 1
PMID 1104896
Citations 3
Authors
Y Yamada
P A Whitaker
D Nakada
Affiliations
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Abstract

T7 early mRNA produced by a gene 1 amber mutant phage, T7 am27, is chemically stable interms of acid insolubility and T7 DNA hybridizability. However, the messenger activity of individual T7 early mRNA species, transcripts of gene 1, gene 0.7, and gene 1.3, decay with a half-life of about 6.5 min at 30 C. An extensive secondary structure is present in all T7 early mRNA species and is probably responsible for the chemical stability of the RNAs after the loss of functional activity. It is unlikely that ribosomes protect T7 early mRNA from nucleolytic degradation.

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Bacteriophage T3 and bacteriophage T7 virus-host cell interactions.

Kruger D, Schroeder C Microbiol Rev. 1981; 45(1):9-51.

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Translation of T7 RNA in vitro without cleavage by RNase III.

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References
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