Evidence for Endonucleolytic Cleavage at the 5'-proximal Segment of the Trp Messenger RNA in Escherichia Coli

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1979 Apr 17

PMID 377022

Citations 7

Authors

Y Kano

F Imamoto

Affiliations

Soon will be listed here.

Abstract

The 5'-proximal trp leader RNA segment (about 5S) decays at 2 to 3 times slower rates than the distal trp mRNA sequence. This has been demonstrated by employing the deletion mutants which lack a large portion of the structural genes but retain the promoter-proximal region of the trp operon. Relative stability of the leader RNA is not merely due to the presence of an untranslatable region in the segment; the internal untranslatable segment of trp mRNA downstream from the nonsense alteration site of a double mutant trpAD28.trpE9758 decays as fast as the normal trp mRNA sequence. These results suggest that the trp mRNA is endonucleolytically cleaved to yield the small 5'-proximal leader RNA segment before the distal mRNA decays and that the leader RNA sequence is not subject to usual mode of mRNA decay in the 5' to 3' direction.

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