Regulation of Glycerol and Maltose Uptake by the IIAGlc-like Domain of IINag of the Phosphotransferase System in Salmonella Typhimurium LT2

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1995 Jul 28

PMID 7651347

Citations 5

Authors

J van der Vlag

P W Postma

Affiliations

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Abstract

In Enterobacteriaceae the nonphosphorylated form of IIAGlc of the phosphoenolpyruvate-dependent phosphotransferase system (PTS) can inhibit the uptake and subsequent metabolism of glycerol and maltose by binding to, and inhibiting, glycerol kinase and the MalK protein of the maltose transport system, respectively. In this report we show that the IIAGlc-like domain of the membrane-bound IIN-acetylglucosamine (IINag) of the PTS can replace IIAGlc in a Salmonella typhimurium crr mutant strain that lacks all soluble IIAGlc. The inhibition was most severe in cells which were partially induced for the glycerol or maltose uptake systems. The Streptococcus thermophilus lactose transporter LacS, which also contains a IIAGlc-like domain, could not replace IIAGlc. Neither IINag nor LacS could replace IIAGlc in activation of adenylate cyclase.

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Crystal structure of MalK, the ATPase subunit of the trehalose/maltose ABC transporter of the archaeon Thermococcus litoralis.

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PMID: 11080142 PMC: 305842. DOI: 10.1093/emboj/19.22.5951.

Phosphorylation and functional properties of the IIA domain of the lactose transport protein of Streptococcus thermophilus.

Gunnewijk M, Postma P, Poolman B J Bacteriol. 1999; 181(2):632-41.

PMID: 9882680 PMC: 93420. DOI: 10.1128/JB.181.2.632-641.1999.

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