Comparison of the Sequences of the NagE Operons from Klebsiella Pneumoniae and Escherichia Coli K12: Enhanced Variability of the Enzyme IIN-acetylglucosamine in Regions Connecting Functional Domains

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1991 Nov 1

PMID 1745234

Citations 9

Authors

A P Vogler

J W Lengeler

Affiliations

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Abstract

The nagE operon, encoding the enzyme II specific for N-acetylglucosamine (EIINag), and adjacent DNA from the chromosome of Klebsiella pneumoniae were sequenced and compared with the corresponding sequence from Escherichia coli K12. The deduced EIINag sequences differ in 72 out of 651 amino acids, the K. pneumoniae sequence being three residues longer. The amino acid differences were distributed unevenly, and were most frequent in regions connecting the three functional domains of the protein. In the nagE-nagB intergenic region, two promoter, two operator, and one CAP consensus sequence with regulatory functions were highly conserved. The nag structural genes from both species were very similar (83% DNA similarity; 89% amino acid similarity) except for frequent AT to GC exchanges in the wobble base of codons in K. pneumoniae DNA relative to the E. coli DNA.

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Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae and plasmid pUR400, which encode the sucrose transport protein Enzyme II Scr of the phosphotransferase system and a sucrose-6-phosphate invertase.

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