Dimerization of Soluble Major Histocompatibility Complex-peptide Complexes is Sufficient for Activation of T Cell Hybridoma and Induction of Unresponsiveness

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 1995 Aug 1

PMID 7629504

Citations 26

Authors

J P Abastado

Y C Lone

A Casrouge

G Boulot

P Kourilsky

Affiliations

Soon will be listed here.

Abstract

Major histocompatibility complex (MHC) class I molecules are cell-surface proteins that present peptides to CD8+ T cells. These peptides are mostly derived from endogenously synthesized protein. Recombinant, soluble MHC class I molecules were produced, purified, and loaded homogeneously with synthetic peptide. These MHC-peptide complexes were used to activate a T cell hybridoma. While monomers of MHC-peptide bound to the T cell, they showed no stimulatory activity. Dimers fully triggered the T cell hybridoma to secrete interleukin 2. This response was followed by a state in which the T cell was refractory to restimulation as a result of defective signal transduction through the T cell receptor.

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