Class I Negative CD8 T Cells Reveal the Confounding Role of Peptide-transfer Onto CD8 T Cells Stimulated with Soluble H2-Kb Molecules

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2002 Oct 11

PMID 12374858

Citations 26

Authors

Eckart Schott

Nicolas Bertho

Qing Ge

Madelon M Maurice

Hidde L Ploegh

Affiliations

Soon will be listed here.

Abstract

Crosslinking of the T cell receptor has been proposed to be a prerequisite for T cell activation. Although the evidence supports this notion for CD4 T cells, the situation for CD8 T cells is less clear. Soluble class I monomers have been used to determine activation requirements in vitro with contradictory results. The possibility of transfer of peptide from soluble class I molecules onto class I molecules present on the surface of CD8 T cells, with ensuing presentation to other CD8 T cells, has been widely ignored. We show that monomeric and tetrameric class I molecules as well as free peptide can stimulate naive CD8 T cells in vitro. We generate and characterize CD8 T cells that express the OT-I T cell receptor (for K(b)/SIINFEKL) yet lack K(b) and D(b) molecules, and show that their activation requirements differ from their class I positive counterparts when stimulated with soluble K(b) molecules. By eliminating the confounding effect of peptide transfer, we unmask the true activation requirements for naive CD8 T cells and show that multivalent engagement of T cell receptors, as well as costimulation, is required for optimal stimulation.

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