The Regulation of the Immune Response of Mice to Haemophilus Influenzae Type B Capsular Polysaccharide

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1978 Jan 1

PMID 75178

Citations 6

Authors

C J Lee

F G Malik

J B Robbins

Affiliations

Soon will be listed here.

Abstract

The regulation of age-related antibody response to Haemophilus influenzae type b polysaccharide (HITB-PS) was studied by measuring the splenic plaque forming cells (PFC) following immunization with this capsular polysaccharide. The magnitude of PFC response to HITB-PS was found to be dose-related, enhanced by Freund's complete adjuvant and influenced by the genetic strain of mice. Priming with a low dose of HITB-PS did not induce a state of immunological unresponsiveness. Treatment with antilymphocyte serum significantly increased the PFC response to HITB-PS. Athymic nude mice showed an enhanced ability to induce both IgG and IgA-PFC responses as well as a significant increase in the biosynthesis of protein and mitogenicity in spleen cells. These findings suggest that the immune response to HITB-PS is regulated by the suppressor T cell. The magnitude of the IgM-PFC response induced by HITB-PS in mice increased gradually from two weeks of age and reached a plateau at 8 weeks. Treatment with fetuin resulted in the inhibition of direct IgM and IgG-PFC responses to HITB-PS; the suppressive effect on the immune response was more profound and lasting in young than in adult mice.

Citing Articles

Maternal-foetal interaction, antibody formation, and metabolic response in mice immunized with pneumococcal polysacharides.

Lee C Immunology. 1980; 41(1):45-54.

PMID: 7429553 PMC: 1458251.

Immunoglobulin M and G antibody responses and persistence of these antibodies in adults after vaccination with a combined meningococcal group A and group C polysaccharide vaccine.

Beuvery E, Leussink A, Van Delft R, Tiesjema R, Nagel J Infect Immun. 1982; 37(2):579-85.

PMID: 6811434 PMC: 347572. DOI: 10.1128/iai.37.2.579-585.1982.

Characterization of the murine immune response to type 6 pneumococcal polysaccharide.

Fairchild R, Braley-Mullen H Infect Immun. 1983; 39(2):615-22.

PMID: 6339385 PMC: 347996. DOI: 10.1128/iai.39.2.615-622.1983.

Evolution of the subclass of IgG antibody to type 3 pneumococcal polysaccharide during childhood.

Schatz D, Barrett D Clin Exp Immunol. 1987; 70(2):449-55.

PMID: 3427829 PMC: 1542071.

T-cell modulation of the murine antibody response to Neisseria meningitidis group A capsular polysaccharide.

Muller E, Apicella M Infect Immun. 1988; 56(1):259-66.

PMID: 3121512 PMC: 259266. DOI: 10.1128/iai.56.1.259-266.1988.

References

Mosier D, Johnson B . Ontogeny of mouse lymphocyte function. II. Development of the ability to produce antibody is modulated by T lymphocytes. J Exp Med. 1975; 141(1):216-26. PMC: 2190507. DOI: 10.1084/jem.141.1.216. View

Olding L, Oldstone B . Thymus-derived peripheral lymphocytes from human newborns inhibit division of their mothers' lymphocytes. J Immunol. 1976; 116(3):682-6. View

Morse 3rd H, PRESCOTT B, Cross S, STASHAK P, Baker P . Regulation of the antibody response to type III pneumococcal polysaccharide. V. Ontogeny of factors influencing the magnitude of the plaque-forming cell response. J Immunol. 1976; 116(2):279-87. View

Pantelouris E . Observations on the immunobiology of 'nude' mice. Immunology. 1971; 20(2):247-52. PMC: 1455799. View

Robbins J, Parke Jr J, Schneerson R, Whisnant J . Quantitative measurement of "natural" and immunization-induced Haemophilus influenzae type b capsular polysaccharide antibodies. Pediatr Res. 1973; 7(3):103-10. DOI: 10.1203/00006450-197303000-00001. View

Barthold D, PRESCOTT B, STASHAK P, Amsbaugh D, Baker P . Regulation of the antibody response to type 3 pneumococcal polysaccharide. 3. Role of regulatory T cells in the development of an IgG and IgA antibody response. J Immunol. 1974; 112(3):1042-50. View

Baker P, STASHAK P, Amsbaugh D, PRESCOTT B . Regulation of the antibody response to type 3 pneumococcal polysaccharide. II. Mode of action of thymic-derived suppressor cells. J Immunol. 1974; 112(1):404-9. View

Baker P, STASHAK P, Amsbaugh D, PRESCOTT B . Regulation of the antibody response to type 3 pneumococcal polysaccharide. IV. Role of suppressor T cells in the development of low-dose paralysis. J Immunol. 1974; 112(6):2020-7. View

Gasser D, Silvers W . Genetic determinants of immunological responsiveness. Adv Immunol. 1974; 18:1-66. DOI: 10.1016/s0065-2776(08)60307-7. View

10.

Baker P, STASHAK P, PRESCOTT B . Use of erythrocytes sensitized with purified pneumococcal polysaccharides for the assay of antibody and antibody-producing cells. Appl Microbiol. 1969; 17(3):422-6. PMC: 377705. DOI: 10.1128/am.17.3.422-426.1969. View

11.

GERSHON R, Cohen P, Hencin R, Liebhaber S . Suppressor T cells. J Immunol. 1972; 108(3):586-90. View

12.

Amsbaugh D, Hansen C, PRESCOTT B, STASHAK P, Barthold D, Baker P . Genetic control of the antibody response to type 3 pneumococcal polysaccharide in mice. I. Evidence that an X-linked gene plays a decisive role in determining responsiveness. J Exp Med. 1972; 136(4):931-49. PMC: 2139279. DOI: 10.1084/jem.136.4.931. View

13.

Anderson P, Peter G, JOHNSTON Jr R, WETTERLOW L, Smith D . Immunization of humans with polyribophosphate, the capsular antigen of Hemophilus influenzae, type b. J Clin Invest. 1972; 51(1):39-44. PMC: 332926. DOI: 10.1172/JCI106794. View

14.

Smith D, Peter G, INGRAM D, Harding A, Anderson P . Responses of children immunized with the capsular polysaccharide of Hemophilus influenzae, type b. Pediatrics. 1973; 52(5):637-44. View

15.

GERSHON R . T cell control of antibody production. Contemp Top Immunobiol. 1974; 3:1-40. DOI: 10.1007/978-1-4684-3045-5_1. View

16.

Wortis H . Immunological studies of nude mice. Contemp Top Immunobiol. 1974; 3:243-63. DOI: 10.1007/978-1-4684-3045-5_10. View

17.

Murgita R, TOMASI Jr T . Suppression of the immune response by alpha-fetoprotein on the primary and secondary antibody response. J Exp Med. 1975; 141(2):269-86. PMC: 2190533. DOI: 10.1084/jem.141.2.269. View

18.

Rodrigues L, Schneerson R, Robbins J . Immunity to Hemophilus influenzae type b. I. The isolation, and some physicochemical, serologic and biologic properties of the capsular polysaccharide of Hemophilus influenzae type b. J Immunol. 1971; 107(4):1071-80. View

19.

Schneerson R, Rodrigues L, Parke Jr J, Robbins J . Immunity to disease caused by Hemophilus influenzae type b. II. Specificity and some biologic characteristics of "natural," infection-acquired, and immunization-induced antibodies to the capsular polysaccharide of Hemophilus influenzae type b. J Immunol. 1971; 107(4):1081-9. View