Uptake of Piperidine and Pipecolic Acid by Synaptosomes from Mouse Brain

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1980 Nov 1

PMID 7464982

Citations 14

Authors

Y Nomura

T Schmidt-Glenewinkel

E Giacobini

Affiliations

Soon will be listed here.

Abstract

Piperidine is actively transported into the synaptosomal fraction of adult mouse brain. The transport mechanism appears to be Na+ independent but is temperature dependent and sensitive to ouabain. Analysis of kinetic experiments indicates only a "low-affinity" transport system to be present. By contrast the uptake of D,L-[(3)H]pipecolic acid at a concentration of 4 X 10(-7)M was temperature and Na+ dependent, ouabain sensitive, and revealed a two-component system with a Km = 3.9 plus or minus 0.17 X 10(-6)M, Vmax = 129 plus or minus 6 pmol/mg protein/3 min for the "high-affinity" system and a Km = 90.2 plus or minus 4.3 X 10 (-6)M, Vmax = 2.45 plus or minus 0.19 nmol/mg protein/3 min for the "low-affinity" system. Compounds structurally related to pipecolic acid such as glycine, L-proline, 4-amino-n-butyric acid, and 5-amino-n-valeric acid showed an inhibitory effect on uptake at a concentration of 10(-4)M. The demonstration of biosynthesis of pipecolic acid in mouse brain and the presence of a "high-affinity" sodium-dependent uptake system suggest a physiological role of this substance in the central nervous system.

Citing Articles

Reciprocal Control of Thyroid Binding and the Pipecolate Pathway in the Brain.

Hallen A, Cooper A Neurochem Res. 2016; 42(1):217-243.

PMID: 27518089 DOI: 10.1007/s11064-016-2015-9.

Accumulation and metabolism of pipecolic acid in the brain and other organs of the mouse.

Nishio H, Ortiz J, Giacobini E Neurochem Res. 1981; 6(12):1241-52.

PMID: 7339504 DOI: 10.1007/BF00964346.

Comparison of synaptosomal and glial uptake of pipecolic acid and GABA in rat brain.

Nomura Y, Okuma Y, Segawa T, Schmidt-Glenewinkel T, Giacobini E Neurochem Res. 1981; 6(4):391-400.

PMID: 7266747 DOI: 10.1007/BF00963854.

Accumulation, elimination, release and metabolism of pipecolic acid in the mouse brain following intraventricular injection.

Nishio H, Giacobini E, Ortiz J Neurochem Res. 1982; 7(4):373-85.

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Lysine metabolism in the human and the monkey: demonstration of pipecolic acid formation in the brain and other organs.

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