Comparison of Synaptosomal and Glial Uptake of Pipecolic Acid and GABA in Rat Brain

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1981 Apr 1

PMID 7266747

Citations 9

Authors

Y Nomura

Y Okuma

T Segawa

T Schmidt-Glenewinkel

E Giacobini

Affiliations

Soon will be listed here.

Abstract

The active uptake of [3H]pipecolic acid increased with incubation time and its uptake at 3 min was half of that at 20 min. On the other hand, a ratio (pellet/medium) of [3H]pipecolic acid uptake into glial cell-enriched fractions, was much less (0.4 - 0.6) than that of [14C]GABA (25.8 - 74.1). GABA, 10(-4) M, and pipecolic acid, 10(-4) M, produced a significant inhibitor of [3H]pipecolic acid uptake into P2 fractions. Pipecolic acid, 10(-4) M, significantly reduced the synaptosomal and glial uptake of [14C]GABA. GABA, 10(-4) M, affected neither spontaneous nor high K+-induced release of [3H]pipecolic acid from brain slices. It is suggested that pipecolic acid is involved in either synaptic transmission or in its modulation at GABA synapses in the central nervous system.

Citing Articles

919 Syrup Alleviates Postpartum Depression by Modulating the Structure and Metabolism of Gut Microbes and Affecting the Function of the Hippocampal GABA/Glutamate System.

Tian X, Xing J, Zheng Q, Gao P Front Cell Infect Microbiol. 2021; 11:694443.

PMID: 34490139 PMC: 8417790. DOI: 10.3389/fcimb.2021.694443.

Hippocampal Sector-Specific Metabolic Profiles Reflect Endogenous Strategy for Ischemia-Reperfusion Insult Resistance.

Krupska O, Kowalczyk T, Beresewicz-Haller M, Samczuk P, Pietrowska K, Zablocki K Mol Neurobiol. 2020; 58(4):1621-1633.

PMID: 33222147 PMC: 7932963. DOI: 10.1007/s12035-020-02208-6.

Insights into physiological roles of unique metabolites released from Plasmodium-infected RBCs and their potential as clinical biomarkers for malaria.

Beri D, Ramdani G, Balan B, Gadara D, Poojary M, Momeux L Sci Rep. 2019; 9(1):2875.

PMID: 30814599 PMC: 6393545. DOI: 10.1038/s41598-018-37816-9.

Metabolic profiling framework for discovery of candidate diagnostic markers of malaria.

Tritten L, Keiser J, Godejohann M, Utzinger J, Vargas M, Beckonert O Sci Rep. 2013; 3:2769.

PMID: 24067624 PMC: 6505674. DOI: 10.1038/srep02769.

Identification of L-amino acid/L-lysine alpha-amino oxidase in mouse brain.

Murthy S, Janardanasarma M Mol Cell Biochem. 1999; 197(1-2):13-23.

PMID: 10485319 DOI: 10.1023/a:1006906505745.

References

Chang Y . Lysine metabolism in the rat brain: the pipecolic acid-forming pathway. J Neurochem. 1978; 30(2):347-54. DOI: 10.1111/j.1471-4159.1978.tb06536.x. View

Martin D . Kinetics of the sodium-dependent transport of gamma-aminobutyric acid by synaptosomes. J Neurochem. 1973; 21(2):345-56. DOI: 10.1111/j.1471-4159.1973.tb04255.x. View

Levi G, Raiteri M . Detectability of high and low affinity uptake systems for GABA and glutamate in rat brain slices and synaptosomes. Life Sci I. 1973; 12(2):81-8. DOI: 10.1016/0024-3205(73)90064-7. View

Minchin M . Factors influencing the efflux of [3H]gamma-aminobutyric acid from satellite glial cells in rat sensory ganglia. J Neurochem. 1975; 24(3):571-7. DOI: 10.1111/j.1471-4159.1975.tb07676.x. View

Johnston G, Stephanson A, Twitchin B . Uptake and release of nipecotic acid by rat brain slices. J Neurochem. 1976; 26(1):83-7. View

Larsson O, Krogsgaard-Larsen P, Schousboe A . High-affinity uptake of (RS)-nipecotic acid in astrocytes cultured from mouse brain. Comparison with GABA transport. J Neurochem. 1980; 34(4):970-7. DOI: 10.1111/j.1471-4159.1980.tb09673.x. View

Nomura Y, Schmidt-Glenewinkel T, Giacobini E . Uptake of piperidine and pipecolic acid by synaptosomes from mouse brain. Neurochem Res. 1980; 5(11):1163-73. DOI: 10.1007/BF00964896. View

Chang Y . Pipecolic acid pathway: the major lysine metabolic route in the rat brain. Biochem Biophys Res Commun. 1976; 69(1):174-80. DOI: 10.1016/s0006-291x(76)80288-4. View

Kase Y, Kataoka M, Miyata T, Okano Y . Pipecolic acid in the dog brain. Life Sci. 1973; 13(7):867-73. DOI: 10.1016/0024-3205(73)90077-5. View

10.

Hernandez M, Chang Y . In vitro synthesis of L-pipecolate from L-lysine: inconsistent with epsilon-N-acetyl-L-lysine as an obligatory intermediate. Biochem Biophys Res Commun. 1980; 93(3):762-9. DOI: 10.1016/0006-291x(80)91142-0. View

11.

Nomura Y, Okuma Y, Segawa T, Schmidt-Glenewinkel T, Giacobini E . A calcium-dependent, high potassium-induced release of pipecolic acid from rat brain slices. J Neurochem. 1979; 33(3):803-5. DOI: 10.1111/j.1471-4159.1979.tb05228.x. View

12.

Minchin M, Iversen L . Release of (3H)gamma-aminobutyric acid from glial cells in rat dorsal root ganglia. J Neurochem. 1974; 23(3):533-40. DOI: 10.1111/j.1471-4159.1974.tb06056.x. View

13.

Chang Y . Lysine metabolism in the rat brain: blood-brain barrier transport, formation of pipecolic acid and human hyperpipecolatemia. J Neurochem. 1978; 30(2):355-60. DOI: 10.1111/j.1471-4159.1978.tb06537.x. View

14.

Schmidt-Glenewinkel T, Nomura Y, Giacobini E . The conversion of lysine into piperidine, cadaverine, and pipecolic acid in the brain and other organs of the mouse. Neurochem Res. 2013; 2(6):619-37. DOI: 10.1007/BF00963776. View

15.

Schousboe A, Krogsgaard-Larsen P, Svenneby G, Hertz L . Inhibition of the high-affinity, net uptake of GABA into cultured astrocytes by beta-proline, nipecotic acid and other compounds. Brain Res. 1978; 153(3):623-6. DOI: 10.1016/0006-8993(78)90349-9. View

16.

Johnston G, Krogsgaard-Larsen P, Stephanson A, Twitchin B . Inhibition of the uptake of GABA and related amino acids in rat brain slices by the optical isomers of nipecotic acid. J Neurochem. 1976; 26(5):1029-32. DOI: 10.1111/j.1471-4159.1976.tb06488.x. View

17.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

18.

Hitzemann R, Loh H . A comparison of GABA and beta-alanine transport and GABA membrane binding in the rat brain. J Neurochem. 1978; 30(2):471-7. DOI: 10.1111/j.1471-4159.1978.tb06552.x. View

19.

Segawa T, Nakata Y, Yajima H, Kitagawa K . Further observation on the lack of active uptake system for substance P in the central nervous system. Jpn J Pharmacol. 1977; 27(4):573-80. DOI: 10.1254/jjp.27.573. View

20.

Henn F, Hamberger A . Glial cell function: uptake of transmitter substances. Proc Natl Acad Sci U S A. 1971; 68(11):2686-90. PMC: 389501. DOI: 10.1073/pnas.68.11.2686. View