Synergistic Action of Cysteamine and BrdU-substituted DNA in the Induction of Sister Chromatid Exchanges

Overview

Journal Chromosoma

Specialty Molecular Biology

Date 1980 Jan 1

PMID 7449571

Citations 4

Authors

G Speit

M Wolf

W Vogel

Affiliations

Soon will be listed here.

Abstract

The effect of different BrdU-concentrations on the cysteamine-induced SCE-rate was investigated in V-79 Chinese hamster cell monolayer cultures. Both cysteamine and its auto-oxidation product cystamine act synergistically with BrdU in the induction of SCEs. A given concentration of these substances produces a low SCE-frequency at low BrdU-concentrations -- but the incidence of SCEs is significantly increased at increased BrdU-concentrations. -- Using one-cycle substitution experiments and the determination of the relative level of substitution by means of 3HBrdU-incorporation, this synergism was shown to depend on the BrdU incorporated in the DNA and on the extent to which this incorporation takes place.

Citing Articles

Demonstration of sister chromatid differentiation in human amniotic fluid cells after partial synchronization of BrdU or dT surplus.

Speit G, Vogel W Hum Genet. 1981; 58(3):298-301.

PMID: 7035332 DOI: 10.1007/BF00294927.

Induction of endoreduplication by hydrazine in Chinese hamster V 79 cells and reduced incidence of sister chromatid exchanges in endoreduplicated mitoses.

Speit G, Mehnert K, Vogel W Chromosoma. 1984; 89(1):79-84.

PMID: 6697818 DOI: 10.1007/BF00302355.

Effect of 5-bromodeoxyuridine substitution on sister chromatid exchange induction by chemicals.

Morgan W, Wolff S Chromosoma. 1984; 89(4):285-9.

PMID: 6204821 DOI: 10.1007/BF00292476.

Detection of bromodeoxyuridine incorporation in mammalian chromosomes by a bromodeoxyuridine antibody. II. Demonstration of sister chromatid exchanges.

Speit G, Vogel W Chromosoma. 1986; 94(2):103-6.

PMID: 3093173 DOI: 10.1007/BF00286987.

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