Induction of Sister Chromatid Exchanges by Hydroxylamine, Hydrazine and Isoniazid and Their Inhibition by Cysteine

Overview

Journal Hum Genet

Specialty Genetics

Date 1980 Jan 1

PMID 7390490

Citations 5

Authors

G Speit

C Wick

M Wolf

Affiliations

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Abstract

Experiments were performed in order to gain information about the primary process leading to the production of sister chromatid exchanges (SCEs). Radical-forming substances (hydroxylamine, hydrazine and the antituberculous drug isoniazid) were examined for their effectiveness in inducing SCEs. All three substances proved successful in the induction of SCEs in the V-79 cell line of the Chinese hamster. By simultaneous application of a sulfhydryl compound (cysteine), a reduction of the hydrazine- and isoniazid-induced SCEs was achieved. Isoniazid was additionally examined in the in vivo SCE-test. At concentrations of 2-100 mg/kg body weight, it does not increase the rate of SCEs in the bone marrow of the Chinese hamster.

Citing Articles

Synergistic action of cysteamine and BrdU-substituted DNA in the induction of sister chromatid exchanges.

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Induction of endoreduplication by hydrazine in Chinese hamster V 79 cells and reduced incidence of sister chromatid exchanges in endoreduplicated mitoses.

Speit G, Mehnert K, Vogel W Chromosoma. 1984; 89(1):79-84.

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Sister chromatid exchange (SCE) and structural chromosome aberration in mutagenicity testing.

Gebhart E Hum Genet. 1981; 58(3):235-54.

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References

Gebhart E . [The effect of L-cysteine on the induction of human chromosome aberrations by chemical mutagens. II. Significance of the time factor with trenimone as aberration-inducing agent]. Humangenetik. 1971; 11(3):237-43. DOI: 10.1007/BF00274743. View

Allen J, Shuler C, LATT S . Bromodeoxyuridine tablet methodology for in vivo studies of DNA synthesis. Somatic Cell Genet. 1978; 4(4):393-405. DOI: 10.1007/BF01538862. View

SOMERS C, Hsu T . Chromosome damage induced by hydroxylamine in mammalian cells. Proc Natl Acad Sci U S A. 1962; 48:937-43. PMC: 220884. DOI: 10.1073/pnas.48.6.937. View

Sasaki M, Matsubara S . Free radical scavenging in protection of human lymphocytes against chromosome aberration formation by gamma-ray irradiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1977; 32(5):439-45. DOI: 10.1080/09553007714551191. View

Epplen J, Siebers J, Vogel W . DNA replication patterns of human chromosomes from fibroblasts and amniotic fluid cells revealed by a Giemsa staining technique. Cytogenet Cell Genet. 1975; 15(3):177-85. DOI: 10.1159/000130516. View

Engel W, Krone W, Wolf U . [The effects of thioguanine, hydroxylamine and 5-bromodeoxyuridine on human chromosomes in vitro]. Mutat Res. 1967; 4(3):353-68. DOI: 10.1016/0027-5107(67)90030-9. View

Perry P, Evans H . Cytological detection of mutagen-carcinogen exposure by sister chromatid exchange. Nature. 1975; 258(5531):121-5. DOI: 10.1038/258121a0. View

Gebhart E . [The effect of L-cysteine on the induction of aberrations by chemical mutagens in human chromosomes. I. Dose-effect relations with Trenimone as aberration-inducing agent]. Humangenetik. 1970; 10(2):115-26. DOI: 10.1007/BF00295510. View

Wolff S . Sister chromatid exchange. Annu Rev Genet. 1977; 11:183-201. DOI: 10.1146/annurev.ge.11.120177.001151. View

10.

Basler A . Analysis of chromosome aberrations in bone marrow cells of rats after treatment with isoniazid. Hum Genet. 1978; 42(1):26-7. DOI: 10.1007/BF00291619. View

11.

BAUCHINGER M, Gebhart E, Fonatsch C, Schmid E, Muller W, Obe G . Chromosome analyses in man in the course of chemoprophylaxis against tuberculosis and of antituberculosis chemotherapy with isoniazid. Hum Genet. 1978; 42(1):31-43. DOI: 10.1007/BF00291621. View

12.

Muller D, Langauer M, Grafe A . Nucleus anomaly test in Chinese hamster and in rat after treatment with isoniazid. Hum Genet. 1978; 42(1):9-14. DOI: 10.1007/BF00291617. View

13.

Speit G, Vogel W . The effect on sister-chromatid exchanges of drugs and dyes by intercalation and photoactivation. Mutat Res. 1979; 59(2):223-9. DOI: 10.1016/0027-5107(79)90161-1. View

14.

Muller D, STRASSER F, ROHRBORN G, Franz J, Grafe A, Mittenburger H . Chromosome analysis of bone marrow in mammals after treatment with isoniazid. Hum Genet. 1978; 42(1):15-25. DOI: 10.1007/BF00291618. View

15.

ROHRBORN G, Miltenburger H, RADENBACH K . A correlated study of the cytogenetic effect of Isoniazid (INH) on cell systems of mammals and man conducted by thirteen laboratories. Introductory remarks. Hum Genet. 1978; 42(1):3-7. DOI: 10.1007/BF00291615. View

16.

Schneider E, Tice R, Kram D . Bromodeoxyuridine differential chromatid staining technique: a new approach to examining sister chromatid exchange and cell replication kinetics. Methods Cell Biol. 1978; 20:379-409. DOI: 10.1016/s0091-679x(08)62029-4. View

17.

BROWN P . Mechanism of action of aminothiol radioprotectors. Nature. 1967; 213(5074):363-4. DOI: 10.1038/213363a0. View

18.

Rosin M, Stich H . Inhibitory effect of reducing agents on N-acetoxy- and N-hydroxy-2-acetylaminofluorene-induced mutagenesis. Cancer Res. 1978; 38(5):1307-10. View

19.

Gebhart E . [L-cysteine as a protector against the aberration-causing effect of 8-hydroxyquinoline sulfate in human leukocyte chromosomes]. Mutat Res. 1971; 11(2):261-2. View