LBA Technique in the Detection of Chromosome Variants. I. Chromosomes with Known Sites of Q Variants

Overview

Journal Hum Genet

Specialty Genetics

Date 1977 Nov 2

PMID 72722

Citations 4

Authors

S Oka

Y NAKAGOME

E Matsunaga

M Arima

Affiliations

Soon will be listed here.

Abstract

A method is described (LBA method) which used DNA replication pattern in the detection of chromosome variants in man. In Part I, results on chromosomes with known sites of Q-variants, i.e., 5 pairs of acrocentrics, as well as 3 and 4, were presented. Fourty-one variants were detected in a total of 40 acrocentrics. Twenty-eight of them were detected only by the LBA technique; 11 of them being in short arms and 17 of them in centromeres. Seven variants, including 4 of those in satellites, were detected only in QFQ-stained metaphases. Six short-arm variants were observed by both methods. It appears that a sequential QFQ-LBA technique is very useful in the detection of variants in D- or G-group chromosomes.

Citing Articles

A new approach in the evaluation of chromosome variants in man. II. Pairs without Q or C (qh) variants.

Azumi J, NAKAGOME Y, Oka S, Matsunaga E Hum Genet. 1980; 55(1):75-9.

PMID: 7450758 DOI: 10.1007/BF00329130.

The genetic structure of the Kuwaiti population II: The distribution of Q-band chromosomal heteromorphisms.

Palmer C, Conneally P, Yu P Hum Genet. 1981; 57(4):423-7.

PMID: 7286984 DOI: 10.1007/BF00281697.

A new approach in the evaluation of chromosome variants in man, III. Pairs with established Q or C variable sites.

Oka S, NAKAGOME Y, Azumi J, Matsunaga E, Igarashi Y Hum Genet. 1980; 55(3):327-31.

PMID: 7203466 DOI: 10.1007/BF00290212.

LBA technique in the detection of chromosome variants. II. Chromosomes except for those with Q variants.

NAKAGOME Y, Oka S, Matsunaga E Hum Genet. 1977; 38(3):307-14.

PMID: 914279 DOI: 10.1007/BF00402157.

References

Niikawa N, Kajii T . Sequential Q- and Acridine orange-marker technique. Humangenetik. 1975; 30(1):83-90. DOI: 10.1007/BF00273636. View

Dutrillaux B, Fosse A . [The mechanism of chromosome segmentation induced by BUDR (5-bromodeoxyuridine)]. Ann Genet. 1974; 17(3):207-11. View

LATT S . Microfluorometric detection of deoxyribonucleic acid replication in human metaphase chromosomes. Proc Natl Acad Sci U S A. 1973; 70(12):3395-9. PMC: 427244. DOI: 10.1073/pnas.70.12.3395. View

Verma R, Lubs H . Variation in human acrocentric chromosomes with acridine orange reverse banding. Humangenetik. 1975; 30(3):225-35. DOI: 10.1007/BF00279188. View

NAKAGOME Y . Initiation of DNA replication in human chromosomes. Exp Cell Res. 1977; 106(2):457-61. DOI: 10.1016/0014-4827(77)90203-8. View

BUHLER E, Tsuchimoto T, Kosztolanyi G, Pia Jurik L . Q-banding of human chromosomes after BUdR and BCdR treatment. Hum Genet. 1976; 31(3):309-16. DOI: 10.1007/BF00270860. View

McKenzie W, Lubs H . Human Q and C chromosomal variations: distribution and incidence. Cytogenet Cell Genet. 1975; 14(2):97-115. DOI: 10.1159/000130330. View

Jonasson J, LINDSTEN J, Lundborg R, KISSMEYER-NIELSEN F, Lamm L, Petersen G . HL-A antigens and heteromorphic fluorescence characters of chromosomes in prenatal paternity investigation. Nature. 1972; 236(5345):312-3. DOI: 10.1038/236312a0. View

BUCKTON K, ORiordan M, Jacobs P, Robinson J, Hill R, Evans H . C- and Q-band polymorphisms in the chromosomes of three human populations. Ann Hum Genet. 1976; 40(1):99-112. DOI: 10.1111/j.1469-1809.1976.tb00168.x. View

10.

Langenbeck U, Hansmann I, Hinney B, Honig V . On the origin of the supernumerary chromosome in autosomal trisomies--with special reference to Down's syndrome. A bias in tracing nondisjunction by chromosomal and biochemical polymorphisms. Hum Genet. 1976; 33(2):89-102. DOI: 10.1007/BF00281882. View

11.

Cook P, Robson E, BUCKTON K, Jacobs P, POLANI P . Segregation of genetic markers in families with chromosome polymorphisms and structural rearrangements involving chromosome 1. Ann Hum Genet. 1974; 37(3):261-74. DOI: 10.1111/j.1469-1809.1974.tb01834.x. View

12.

Robinson J . Origin of extra chromosome in trisomy 21. Lancet. 1973; 1(7795):131-3. DOI: 10.1016/s0140-6736(73)90198-0. View

13.

Muller H, Klinger H, Glasser M . Chromosome polymorphism in a human newborn population. II. Potentials of polymorphic chromosome variants for characterizing the idiogram of an individual. Cytogenet Cell Genet. 1975; 15(4):239-55. DOI: 10.1159/000130522. View

14.

CASPERSSON T, LOMAKKA G, ZECH L . The 24 fluorescence patterns of the human metaphase chromosomes - distinguishing characters and variability. Hereditas. 1972; 67(1):89-102. DOI: 10.1111/j.1601-5223.1971.tb02363.x. View

15.

Mikkelsen M, Hallberg A, POULSEN H . Maternal and paternal origin of extra chromosome in trisomy 21. Hum Genet. 1976; 32(1):17-21. DOI: 10.1007/BF00569972. View

16.

Dutrillaux B, Laurent C, Couturier J, LeJeune J . [Staining of human chromosomes with acridine orange after treatment with 5 bromodeoxyuridine]. C R Acad Hebd Seances Acad Sci D. 1973; 276(24):3179-81. View

17.

Sperling K, Kaden R, GILLERT K, Weise W . [A case of XX-XY chimerism with normal male phenotype (author's transl)]. Humangenetik. 1974; 21(3):237-44. DOI: 10.1007/BF00279018. View

18.

NAKAGOME Y, Oka S, Matsunaga E . LBA technique in the detection of chromosome variants. II. Chromosomes except for those with Q variants. Hum Genet. 1977; 38(3):307-14. DOI: 10.1007/BF00402157. View

19.

Wagenbichler P, Killian W, RETT A, Schnedl W . Origin of the extra chromosome no. 21 in Down's syndrome. Hum Genet. 1976; 32(1):13-6. DOI: 10.1007/BF00569971. View

20.

Hauge M, POULSEN H, Halberg A, Mikkelsen M . The value of fluorescence markers in the distinction between maternal and fetal chromosomes. Humangenetik. 1975; 26(3):187-91. DOI: 10.1007/BF00281452. View