Plasmid Specifying Total Degradation of 3-chlorobenzoate by a Modified Ortho Pathway

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 May 1

PMID 7217013

Citations 57

Authors

D K Chatterjee

S T Kellogg

S Hamada

A M Chakrabarty

Affiliations

Soon will be listed here.

Abstract

A plasmid, termed pAC25, specifying biodegradation of 3-chlorobenzoate in a strain of Pseudomonas putida has been characterized. During growth of the plasmid-harboring cells with 3-chlorobenzoate, there was an accumulation of 3-chlorocatechol and beta-chloromuconic acid as intermediates and release of more than 80% of the chlorine in the form of inorganic chloride. The plasmid had a mean molecular mass of 68 x 10(6) daltons and was transmissible to a number of Pseudomonas species such as P. aeruginosa, P. putida strain PpG1, and P. putida strain PRS1. Transfer of pAC25 to various catechol-negative mutants of P. putida strain PRS1 showed that the chromosomally coded pyrocatechase was not complemented by the plasmid-specified pyrocatechase, which appeared to be specific for the chlorinated catechols. In contrast to benzoate, which was metabolized by the ortho pathway through beta-ketoadipate as an intermediate, the plasmid specified ortho cleavage of the chlorocatechols through maleylacetate as an intermediate.

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