Dynamics of Nuclear Actin Bundle Induction by Dimethyl Sulfoxide and Factors Affecting Its Development

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1980 Jan 1

PMID 7188610

Citations 26

Authors

Y Fukui

H Katsumaru

Affiliations

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Abstract

We have reported in a previous paper that dimethyl sulfoxide (DMSO) induces the formation of huge bundles of actin filaments in the nuclei of Dictyostelium mucoroides. The present study was performed to provide electron microscope data on the induction of nuclear actin bundles, illustrating both their formation and their reversion, as well as on the effects of various factors on the induction. The large nuclear bundles of actin appeared after 20--30 min of treatment with 10% DMSO. A DMSO concentration of 5 or 10% was optimal for the induction of the bundles. The nuclear actin bundle reverted to the original morphology within 5 min after removing DMSO. Induction of nuclear actin bundles was inhibited by Mg++ and low temperatures, but not by Na+, K+, Ca++, ATP, 3'5'-cyclic adenosine monophosphate (cAMP), phosphate buffer, or cytochalasin B. Neither NaN3 nor cycloheximide totally inhibited the induction of the bundles.

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References

Ishikawa H, Bischoff R, Holtzer H . Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol. 1969; 43(2):312-28. PMC: 2107852. View

Newell P, Telser A, Sussman M . Alternative developmental pathways determined by environmental conditions in the cellular slime mold Dictyostelium discoideum. J Bacteriol. 1969; 100(2):763-8. PMC: 250156. DOI: 10.1128/jb.100.2.763-768.1969. View

Ryser U . [The ultrastructure of mitotic nuclei in plasmodia of Physarum polycephalum]. Z Zellforsch Mikrosk Anat. 1970; 110(1):108-30. View

Behnke O, Forer A, Emmersen J . Actin in sperm tails and meiotic spindles. Nature. 1971; 234(5329):408-10. DOI: 10.1038/234408a0. View

Pollard T, Weihing R . Actin and myosin and cell movement. CRC Crit Rev Biochem. 1974; 2(1):1-65. DOI: 10.3109/10409237409105443. View

Hinkley R, Telser A . Heavy meromyosin-binding filaments in the mitotic apparatus of mammaliam cells. Exp Cell Res. 1974; 86(1):161-4. DOI: 10.1016/0014-4827(74)90662-4. View

Spudich J . Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. II. Purification, properties, and membrane association of actin from amoebae of Dictyostelium discoideum. J Biol Chem. 1974; 249(18):6013-20. View

Lestourgeon W, Forer A, Yang Y, Bertram J, Pusch H . Contractile proteins. Major components of nuclear and chromosome non-histone proteins. Biochim Biophys Acta. 1975; 379(2):529-52. View

Sanger J . Presence of actin during chromosomal movement. Proc Natl Acad Sci U S A. 1975; 72(6):2451-5. PMC: 432777. DOI: 10.1073/pnas.72.6.2451. View

10.

Spudich J, Cooke R . Supramolecular forms of actin from amoebae of Dictyostelium discoideum. J Biol Chem. 1975; 250(18):7485-91. View

11.

Douvas A, Harrington C, Bonner J . Major nonhistone proteins of rat liver chromatin: preliminary identification of myosin, actin, tubulin, and tropomyosin. Proc Natl Acad Sci U S A. 1975; 72(10):3902-6. PMC: 433104. DOI: 10.1073/pnas.72.10.3902. View

12.

Ellzey J, Huizar E, Yanez D . Microfilament bundles in antheridial nuclei of Achlya ambisexualis E 87. Arch Microbiol. 1976; 107(1):113-4. DOI: 10.1007/BF00427876. View

13.

Tilney L . The polymerization of actin. II. How nonfilamentous actin becomes nonrandomly distributed in sperm: evidence for the association of this actin with membranes. J Cell Biol. 1976; 69(1):51-72. PMC: 2110972. DOI: 10.1083/jcb.69.1.51. View

14.

Tilney L . The polymerization of actin. III. Aggregates of nonfilamentous actin and its associated proteins: a storage form of actin. J Cell Biol. 1976; 69(1):73-89. PMC: 2110965. DOI: 10.1083/jcb.69.1.73. View

15.

Eckert B, WARREN R, Rubin R . Structural and biochemical aspects of cell motility in amebas of Dictyostelium discoideum. J Cell Biol. 1977; 72(2):339-50. PMC: 2111002. DOI: 10.1083/jcb.72.2.339. View

16.

Cande W, Lazarides E, McIntosh J . A comparison of the distribution of actin and tubulin in the mammalian mitotic spindle as seen by indirect immunofluorescence. J Cell Biol. 1977; 72(3):552-67. PMC: 2111019. DOI: 10.1083/jcb.72.3.552. View

17.

Pederson T . Isolation and characterization of chromatin from the cellular slime mold, Dictyostelium discoideum. Biochemistry. 1977; 16(12):2771-7. DOI: 10.1021/bi00631a029. View

18.

Clarke M, Spudich J . Nonmuscle contractile proteins: the role of actin and myosin in cell motility and shape determination. Annu Rev Biochem. 1977; 46:797-822. DOI: 10.1146/annurev.bi.46.070177.004053. View

19.

Condeelis J, Taylor D . The contractile basis of amoeboid movement. V. The control of gelation, solation, and contraction in extracts from Dictyostelium discoideum. J Cell Biol. 1977; 74(3):901-27. PMC: 2110086. DOI: 10.1083/jcb.74.3.901. View

20.

PUCK T . Cyclic AMP, the microtubule-microfilament system, and cancer. Proc Natl Acad Sci U S A. 1977; 74(10):4491-5. PMC: 431969. DOI: 10.1073/pnas.74.10.4491. View