Protein-synthesis Inhibition Induces Perichromatin Granule Accumulation and Intranuclear Rodlet Formation in Osmotically Stimulated Supraoptic Neurons

Overview

Journal Anat Embryol (Berl)

Specialties Anatomy & Histology
Reproductive Medicine

Date 1993 Apr 1

PMID 8512088

Citations 3

Authors

M Lafarga

M T Berciano

M A Andres

Affiliations

Soon will be listed here.

Abstract

The distribution of perichromatin granules (PGs), a storage form of pre-mRNAs, was studied in supraoptic neurons of control and osmotically stimulated rats, and also after treatment with cycloheximide, a protein-synthesis inhibitor. In non-cycloheximide-treated rats, neuronal activation by dehydration significantly decreased the number of PGs. Conversely, PGs were drastically increased in the supraoptic neurons of dehydrated rats treated with cycloheximide for 4 h. This suggests that cycloheximide does not interfere with the transcriptional activation induced by dehydration, but it affects the processing of newly synthesized pre-mRNAs. Moreover, protein-synthesis inhibition was associated with the formation of intranuclear bundles of tubular filaments.

Citing Articles

Nuclei of aged myofibres undergo structural and functional changes suggesting impairment in RNA processing.

Malatesta M, Perdoni F, Muller S, Zancanaro C, Pellicciari C Eur J Histochem. 2009; 53(2):e12.

PMID: 19683983 PMC: 3167280. DOI: 10.4081/ejh.2009.e12.

A long-term study on female mice fed on a genetically modified soybean: effects on liver ageing.

Malatesta M, Boraldi F, Annovi G, Baldelli B, Battistelli S, Biggiogera M Histochem Cell Biol. 2008; 130(5):967-77.

PMID: 18648843 DOI: 10.1007/s00418-008-0476-x.

Reduction of neuronal intranuclear rodlets immunoreactive for tubulin and glucocorticoid receptor in Alzheimer's disease.

Woulfe J, Hammond R, Richardson B, Sooriabalan D, Parks W, Rippstein P Brain Pathol. 2002; 12(3):300-7.

PMID: 12146798 PMC: 8095929. DOI: 10.1111/j.1750-3639.2002.tb00444.x.

References

Welch W, Suhan J . Morphological study of the mammalian stress response: characterization of changes in cytoplasmic organelles, cytoskeleton, and nucleoli, and appearance of intranuclear actin filaments in rat fibroblasts after heat-shock treatment. J Cell Biol. 1985; 101(4):1198-211. PMC: 2113902. DOI: 10.1083/jcb.101.4.1198. View

Scheer U, Hinssen H, Franke W, Jockusch B . Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes. Cell. 1984; 39(1):111-22. DOI: 10.1016/0092-8674(84)90196-x. View

MASUROVSKY E, BENITEZ H, Kim S, Murray M . Origin, development, and nature of intranuclear rodlets and associated bodies in chicken sympathetic neurons. J Cell Biol. 1970; 44(1):172-91. PMC: 2107790. DOI: 10.1083/jcb.44.1.172. View

Sherman T, Day R, Civelli O, Douglass J, Herbert E, Akil H . Regulation of hypothalamic magnocellular neuropeptides and their mRNAs in the Brattleboro rat: coordinate responses to further osmotic challenge. J Neurosci. 1988; 8(10):3785-96. PMC: 6569603. View

Hwang K, Yang L, Carrico C, Schulz R, Schenkman J, Sartorelli A . Production of membrane whorls in rat liver by some inhibitors of protein synthesis. J Cell Biol. 1974; 62(1):20-31. PMC: 2109184. DOI: 10.1083/jcb.62.1.20. View

Tweedle C, Hatton G . Ultrastructural changes in rat hypothalamic neurosecretory cells and their associated glia during minimal dehydration and rehydration. Cell Tissue Res. 1977; 181(1):59-72. DOI: 10.1007/BF00222774. View

PUVION E, Lange M . Functional significance of perichromatin granule accumulation induced by cadmium chloride in isolated rat liver cells. Exp Cell Res. 1980; 128(1):47-58. DOI: 10.1016/0014-4827(80)90385-7. View

Lestourgeon W, Forer A, Yang Y, Bertram J, Pusch H . Contractile proteins. Major components of nuclear and chromosome non-histone proteins. Biochim Biophys Acta. 1975; 379(2):529-52. View

Fukui Y, Katsumaru H . Dynamics of nuclear actin bundle induction by dimethyl sulfoxide and factors affecting its development. J Cell Biol. 1980; 84(1):131-40. PMC: 2110527. DOI: 10.1083/jcb.84.1.131. View

10.

Barondes S . Cerebral protein synthesis inhibitors block long-term memory. Int Rev Neurobiol. 1970; 12:177-205. DOI: 10.1016/s0074-7742(08)60061-6. View

11.

Carmo-Fonseca M, Pepperkok R, Carvalho M, Lamond A . Transcription-dependent colocalization of the U1, U2, U4/U6, and U5 snRNPs in coiled bodies. J Cell Biol. 1992; 117(1):1-14. PMC: 2289407. DOI: 10.1083/jcb.117.1.1. View

12.

Sanger J, Sanger J, Kreis T, Jockusch B . Reversible translocation of cytoplasmic actin into the nucleus caused by dimethyl sulfoxide. Proc Natl Acad Sci U S A. 1980; 77(9):5268-72. PMC: 350039. DOI: 10.1073/pnas.77.9.5268. View

13.

Hatton G . Emerging concepts of structure-function dynamics in adult brain: the hypothalamo-neurohypophysial system. Prog Neurobiol. 1990; 34(6):437-504. DOI: 10.1016/0301-0082(90)90017-b. View

14.

Morris J, Dyball R . A quantitative study of the ultrastructural changes in the hypothalamo-neurohypophysial system during and after experimentally induced hypersecretion. Cell Tissue Res. 1974; 149(4):525-35. DOI: 10.1007/BF00223030. View

15.

SEITE R, Zerbib R, Cataldo C, Escaig J, Pebusque M . Thee-dimensional organization of tubular and filamentous nuclear inclusions and associated structures in sympathetic neurons as revealed by serial sections and tilting experiments. J Ultrastruct Res. 1979; 69(2):211-31. DOI: 10.1016/s0022-5320(79)90111-4. View

16.

Fernandez B, Suarez I, Gutierrez C . Intranuclear inclusions in hypothalamic neurons: a study in the ageing hamster. J Submicrosc Cytol. 1984; 16(3):495-501. View

17.

Bader B, Magin T, Freudenmann M, STUMPP S, Franke W . Intermediate filaments formed de novo from tail-less cytokeratins in the cytoplasm and in the nucleus. J Cell Biol. 1991; 115(5):1293-307. PMC: 2289233. DOI: 10.1083/jcb.115.5.1293. View

18.

Lafarga M, Berciano M, Martinez-Guijarro F, Andres M, Mellstrom B, Lopez-Garcia C . Fos-like expression and nuclear size in osmotically stimulated supraoptic nucleus neurons. Neuroscience. 1992; 50(4):867-75. DOI: 10.1016/0306-4522(92)90210-s. View

19.

Fakan S . Inhibition of nucleolar RNP synthesis by cycloheximide as studied by high resolution radioautography. J Ultrastruct Res. 1971; 34(5):586-96. DOI: 10.1016/s0022-5320(71)80065-5. View

20.

Stoyanova B, HADJIOLOV A . Alterations in the processing of rat-liver ribosomal RNA caused by cycloheximide inhibition of protein synthesis. Eur J Biochem. 1979; 96(2):349-56. DOI: 10.1111/j.1432-1033.1979.tb13046.x. View