A Simple Test for Macromolecular Heterogeneity in the Analytical Ultracentrifuge

Overview

Journal Biochem J

Specialty Biochemistry

Date 1982 Sep 1

PMID 7150237

Citations 6

Authors

J M CREETH

S E Harding

Affiliations

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Abstract

A simple check for the presence of heterogeneity in a macromolecular system is proposed, employing comparison of Rayleigh sedimentation-equilibrium patterns for two solutions of the same fringe concentration but differing absolute concentrations. The method is illustrated by application to a bronchial glycoprotein from a cystic-fibrosis patient.

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References

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Gilbert G, Gilbert L . Detection in the ultracentrifuge of protein heterogeneity by computer modelling, illustrated by pyruvate dehydrogenase multienzyme complex. J Mol Biol. 1980; 144(3):405-8. DOI: 10.1016/0022-2836(80)90099-6. View

YPHANTIS D . EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS. Biochemistry. 1964; 3:297-317. DOI: 10.1021/bi00891a003. View