Primary Structure of the H-2Db Alloantigen. II. Additional Amino Acid Sequence Information, Localization of a Third Site of Glycosylation and Evidence for K and D Region Specific Sequences

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1982 Jan 1

PMID 7118212

Citations 29

Authors

W L Maloy

J E Coligan

Affiliations

Soon will be listed here.

Abstract

The complete amino acid sequence of the CNBr fragment comprising residues 229-284 of the murine major histocompatibility complex antigen H-2Db has been determined using radiochemical methodology. The sequence was determined by N-terminal sequence analysis of the intact CNBr fragment and by sequence determinations of peptides derived from this fragment by trypsin and staphylococcal V8 protease cleavage. In addition to the amino acid assignments for H-2Db, it was possible to assign the linkage position of the third N-linked glycosyl unit to the asparagine at residue 256. Additional amino acid sequence assignments have also been made for three other CNBr fragments that span residues 99-138, 139-228, and 308-331 of the H-2Db molecule. The total protein sequence information available (222 of 338 residues) agrees in every comparable position with the protein sequence derived from the cDNA clone (pH203) isolated by Reyes and co-workers (1982b), which strongly suggests that this clone encodes H-2Db. Combination of the protein sequence with that deduced from the cDNA clone provides the complete H-2Db protein sequence. Comparison of this sequence with other available protein sequence information for murine class I molecules has revealed protein sequences that may be unique to either K or D region molecules.

Citing Articles

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References

Kimball E, Maloy W, Martinko J, Nathenson S, Coligan J . Structural studies on H-2Db and H-2Kd molecules indicate that murine major histocompatibility b and d haplotype alloantigens share structural features. Mol Immunol. 1980; 17(10):1283-91. DOI: 10.1016/0161-5890(80)90025-5. View

Maloy W, Hammerling G, Nathenson S, Coligan J . Comparison of alloantisera and hybridoma antibody for purification of the H-2Db murine histocompatibility antigen and preliminary molecular characterization of this antigen. J Immunol Methods. 1980; 37(3-4):287-99. DOI: 10.1016/0022-1759(80)90314-2. View

Moore K, Sher B, Sun Y, Eakle K, Hood L . DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen. Science. 1982; 215(4533):679-82. DOI: 10.1126/science.7058332. View

Steinmetz M, Frelinger J, Fisher D, Hunkapiller T, Pereira D, Weissman S . Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981; 24(1):125-34. DOI: 10.1016/0092-8674(81)90508-0. View

Rothbard J, Hopp T, Edelman G, Cunningham B . Structure of the heavy chain of the H-2Kk histocompatibility antigen. Proc Natl Acad Sci U S A. 1980; 77(7):4239-43. PMC: 349807. DOI: 10.1073/pnas.77.7.4239. View

Marshall R . Glycoproteins. Annu Rev Biochem. 1972; 41:673-702. DOI: 10.1146/annurev.bi.41.070172.003325. View

Kimball E, Nathenson S, Coligan J . Amino acid sequence of residues 1-98 of the K-2Kb murine major histocompatibility alloantigen: comparison with H-2Kb and H-2db reveals extensive localized differences. Biochemistry. 1981; 20(11):3301-8. DOI: 10.1021/bi00514a049. View

Kimball E, Maloy W, Coligan J . Evidence for three carbohydrate prosthetic groups on mouse histocompatibility antigens H-2Kd and H-2Db. Mol Immunol. 1981; 18(7):677-80. DOI: 10.1016/0161-5890(81)90039-0. View

Maloy W, Nathenson S, Coligan J . Primary structure of murine major histocompatibility complex alloantigens. Amino acid sequence of the NH2-terminal ninety-eight residues of the H-2Db glycoprotein. J Biol Chem. 1981; 256(6):2863-72. View

10.

Uehara H, Ewenstein B, Martinko J, Nathenson S, Coligan J, Kindt T . Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein. Biochemistry. 1980; 19(2):306-15. DOI: 10.1021/bi00543a009. View

11.

Bregegere F, Abastado J, Kvist S, Rask L, Lalanne J, Garoff H . Structure of C-terminal half of two H-2 antigens from cloned mRNA. Nature. 1981; 292(5818):78-81. DOI: 10.1038/292078a0. View

12.

Ploegh H, Orr H, Strominger J . Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules. Cell. 1981; 24(2):287-99. DOI: 10.1016/0092-8674(81)90318-4. View

13.

Steinmetz M, Moore K, Frelinger J, Sher B, Shen F, Boyse E . A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981; 25(3):683-92. DOI: 10.1016/0092-8674(81)90175-6. View

14.

Coligan J, Kindt T, Nairn R, Nathenson S, Sachs D, Hansen T . Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens. Proc Natl Acad Sci U S A. 1980; 77(2):1134-8. PMC: 348439. DOI: 10.1073/pnas.77.2.1134. View

15.

Ewenstein B, Nisizawa T, Uehara H, Nathenson S, Coligan J, Kindt T . Primary structure of murine major histocompatibility complex alloantigens: isolation, biochemical characterization, and preliminary alignment of CNBr fragments from the H-2Ib glycoprotein. Proc Natl Acad Sci U S A. 1978; 75(6):2909-13. PMC: 392675. DOI: 10.1073/pnas.75.6.2909. View

16.

Hansen T, Ozato K, Melino M, Coligan J, Kindt T, Jandinski J . Immunochemical evidence in two haplotypes for at least three D region-encoded molecules, D, L, and R. J Immunol. 1981; 126(5):1713-6. View

17.

Coligan J, Kindt T . Determination of protein primary structure by radiochemical techniques. J Immunol Methods. 1981; 47(1):1-11. DOI: 10.1016/0022-1759(81)90251-9. View

18.

Shearer G . Major histocompatibility complex restricted cell-mediated immunity. Adv Immunol. 1977; 25:55-91. DOI: 10.1016/s0065-2776(08)60931-1. View

19.

Schwartz B, Kato K, Cullen S, Nathenson S . H-2 histocompatibility alloantigens. Some biochemical properties of the molecules solubilized by NP-40 detergent. Biochemistry. 1973; 12(11):2157-64. DOI: 10.1021/bi00735a023. View

20.

Nathenson S, Uehara H, Ewenstein B, Kindt T, Coligan J . Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex. Annu Rev Biochem. 1981; 50:1025-52. DOI: 10.1146/annurev.bi.50.070181.005113. View