The Complete Amino Acid Sequence of the Murine Transplantation Antigen H-2Db As Deduced by Molecular Cloning

Overview

Journal Immunogenetics

Specialties Allergy & Immunology
Genetics

Date 1982 Jan 1

PMID 7118211

Citations 37

Authors

A A Reyes

M Schold

R B Wallace

Affiliations

Soon will be listed here.

Abstract

A mouse cDNA library derived from the EL4 cell line (b-haplotype) was screened with a probe containing a small part of the H-2Kb coding region. One of the clones isolated, pH203, encodes a protein whose deduced amino acid sequence is identical with the known sequence of H-2Db in 141 of 141 positions available for comparison. The clone, therefore, is believed to code for the H-2Db transplantation antigen. The cDNA insert of pH203 contains the coding region for residues 82 through the carboxy-terminus of H-2Db, and includes 476 nucleotides of the 3'-untranslated sequence. Comparison between the H-2Db cDNA clone and a previously isolated H-2Kb cDNA clone shows homologies of 83% and 91% at the amino acid and nucleotide levels, respectively. Analysis of DNA sequences at the 3'-coding and untranslated regions suggests that the mRNAs of H-2Kb and H-2Db are spliced differently at their 3'-coding ends.

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References

Kimball E, Maloy W, Martinko J, Nathenson S, Coligan J . Structural studies on H-2Db and H-2Kd molecules indicate that murine major histocompatibility b and d haplotype alloantigens share structural features. Mol Immunol. 1980; 17(10):1283-91. DOI: 10.1016/0161-5890(80)90025-5. View

Michaelson J, Flaherty L, Vitetta E, Poulik M . Molecular similarities between the Qa-2 alloantigen and other gene products of the 17th chromosome of the mouse. J Exp Med. 1977; 145(4):1066-70. PMC: 2180627. DOI: 10.1084/jem.145.4.1066. View

Steinmetz M, Frelinger J, Fisher D, Hunkapiller T, Pereira D, Weissman S . Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes. Cell. 1981; 24(1):125-34. DOI: 10.1016/0092-8674(81)90508-0. View

Uehara H, Ewenstein B, Martinko J, Nathenson S, Kindt T, Coligan J . Primary structure of murine major histocompatibility alloantigens: amino acid sequence of the cyanogen bromide fragment Ia (positions 139-228) from the H-2Kb molecule. Biochemistry. 1980; 19(26):6182-8. DOI: 10.1021/bi00567a036. View

Marshall R . Glycoproteins. Annu Rev Biochem. 1972; 41:673-702. DOI: 10.1146/annurev.bi.41.070172.003325. View

Strominger J, Orr H, Parham P, Ploegh H, Mann D, Bilofsky H . An evaluation of the significance of amino acid sequence homologies in human histocompatibility antigens (HLA-A and HLA-B) with immunoglobulins and other proteins, using relatively short sequences. Scand J Immunol. 1980; 11(6):573-92. DOI: 10.1111/j.1365-3083.1980.tb00026.x. View

Kimball E, Maloy W, Coligan J . Evidence for three carbohydrate prosthetic groups on mouse histocompatibility antigens H-2Kd and H-2Db. Mol Immunol. 1981; 18(7):677-80. DOI: 10.1016/0161-5890(81)90039-0. View

Maloy W, Nathenson S, Coligan J . Primary structure of murine major histocompatibility complex alloantigens. Amino acid sequence of the NH2-terminal ninety-eight residues of the H-2Db glycoprotein. J Biol Chem. 1981; 256(6):2863-72. View

Maloy W, Coligan J . Primary structure of the H-2Db alloantigen. II. Additional amino acid sequence information, localization of a third site of glycosylation and evidence for K and D region specific sequences. Immunogenetics. 1982; 16(1):11-22. DOI: 10.1007/BF00364438. View

10.

Uehara H, Ewenstein B, Martinko J, Nathenson S, Coligan J, Kindt T . Primary structure of murine major histocompatibility complex alloantigens: amino acid sequence of the amino-terminal one hundred and seventy-three residues of the H-2Kb glycoprotein. Biochemistry. 1980; 19(2):306-15. DOI: 10.1021/bi00543a009. View

11.

Vitetta E, Poulik M, Klein J, UHR J . Beta 2-microglobulin is selectively associated with H-2 and TL alloantigens on murine lymphoid cells. J Exp Med. 1976; 144(1):179-92. PMC: 2190358. DOI: 10.1084/jem.144.1.179. View

12.

Levy R, Hansen T . Functional studies of the products of H-2L locus. Immunogenetics. 1980; 10(1):7-17. DOI: 10.1007/BF01561548. View

13.

Steinmetz M, Moore K, Frelinger J, Sher B, Shen F, Boyse E . A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains. Cell. 1981; 25(3):683-92. DOI: 10.1016/0092-8674(81)90175-6. View

14.

Coligan J, Kindt T, Nairn R, Nathenson S, Sachs D, Hansen T . Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens. Proc Natl Acad Sci U S A. 1980; 77(2):1134-8. PMC: 348439. DOI: 10.1073/pnas.77.2.1134. View

15.

Hansen T, Ozato K, Melino M, Coligan J, Kindt T, Jandinski J . Immunochemical evidence in two haplotypes for at least three D region-encoded molecules, D, L, and R. J Immunol. 1981; 126(5):1713-6. View

16.

Nathenson S, Uehara H, Ewenstein B, Kindt T, Coligan J . Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex. Annu Rev Biochem. 1981; 50:1025-52. DOI: 10.1146/annurev.bi.50.070181.005113. View

17.

Coligan J, Kindt T, Uehara H, Martinko J, Nathenson S . Primary structure of a murine transplantation antigen. Nature. 1981; 291(5810):35-9. DOI: 10.1038/291035a0. View

18.

Bodmer W . New genetic model for allelism at histocompatibility and other complex loci: polymorphism for control of gene expression. Transplant Proc. 1973; 5(4):1471-5. View

19.

Reyes A, Schold M, Itakura K, Wallace R . Isolation of a cDNA clone for the murine transplantation antigen H-2Kb. Proc Natl Acad Sci U S A. 1982; 79(10):3270-4. PMC: 346397. DOI: 10.1073/pnas.79.10.3270. View

20.

Soloski M, UHR J, Flaherty L, Vitetta E . Qa-2, H-2K, and H-2D alloantigens evolved from a common ancestral gene. J Exp Med. 1981; 153(5):1080-93. PMC: 2186164. DOI: 10.1084/jem.153.5.1080. View