Complete Sequence of HLA-B27 CDNA Identified Through the Characterization of Structural Markers Unique to the HLA-A, -B, and -C Allelic Series

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Mar 1

PMID 3485286

Citations 16

Authors

H Szots

G Riethmuller

E Weiss

T Meo

Affiliations

Soon will be listed here.

Abstract

Antigen HLA-B27 is a high-risk genetic factor with respect to a group of rheumatoid disorders, especially ankylosing spondylitis. A cDNA library was constructed from an autozygous B-cell line expressing HLA-B27, HLA-Cw1, and the previously cloned HLA-A2 antigen. Clones detected with an HLA probe were isolated and sorted into homology groups by differential hybridization and restriction maps. Nucleotide sequencing allowed the unambiguous assignment of cDNAs to HLA-A, -B, and -C loci. The HLA-B27 mRNA has the structural features and the codon variability typical of an HLA class I transcript but it specifies two uncommon amino acid replacements: a cysteine in position 67 and a serine in position 131. The latter substitution may have functional consequences, because it occurs in a conserved region and at a position invariably occupied by a species-specific arginine in humans and lysine in mice. The availability of the complete sequence of HLA-B27 and of the partial sequence of HLA-Cw1 allows the recognition of locus-specific sequence markers, particularly, but not exclusively, in the transmembrane and cytoplasmic domains.

Citing Articles

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HLA-B27 and the causes of arthritis: does molecular biology help?.

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Comparison of the primary structure of class I molecules.

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Isolation, expression, and the primary structure of HLA-Cw1 and HLA-Cw2 genes: evolutionary aspects.

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Cloning and analysis of HLA class I cDNA encoding a new HLA-C specificity Cx52.

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