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Evaluation of the Lumac Kit for the Detection of Bacteriuria by Bioluminescence

Overview
Journal J Clin Pathol
Specialty Pathology
Date 1982 Jan 1
PMID 7061712
Citations 12
Authors
D Mackett
S Bascomb
C S Easmon
Affiliations
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Abstract

Four hundred and twenty-two urine samples were screened for significant bacteriuria using bioluminescence and microscopy of uncentrifuged urine. A smaller number of false-negatives were seen with bioluminescence (10%) than with microscopy (40%) while both techniques gave a similar number of false-positives (18%). The kit required a large amount of manual preparation, largely pipetting. With this and the short shelf-life of the reconstituted reagents, it is not suitable for small numbers of urines. At 45p per urine, the cost of bioluminescence is too high.

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References
1.
Bailey R . Significance of coagulase-negative Staphylococcus in urine. J Infect Dis. 1973; 127(2):179-82. DOI: 10.1093/infdis/127.2.179. View
2.
CONN R, CHARACHE P, Chappelle E . Limits of applicability of the firefly luminescence ATP assay for the detection of bacteria in clinical specimens. Am J Clin Pathol. 1975; 63(4):493-501. DOI: 10.1093/ajcp/63.4.493. View
3.
Thore A, Ansehn S, Lundin A, Bergman S . Detection of bacteriuria by luciferase assay of adenosine triphosphate. J Clin Microbiol. 1975; 1(1):1-8. PMC: 274930. DOI: 10.1128/jcm.1.1.1-8.1975. View
4.
Johnston H, Mitchell C, CURTIS G . An automated test for the detection of significant bacteriuria. Lancet. 1976; 2(7982):400-2. DOI: 10.1016/s0140-6736(76)92411-9. View
5.
Barbin G, Thorley J, REINARZ J . Simplified microscopy for rapid detection of significant bacteriuria in random urine specimens. J Clin Microbiol. 1978; 7(3):286-9. PMC: 274916. DOI: 10.1128/jcm.7.3.286-289.1978. View