Reliability of a Bioluminescence ATP Assay for Detection of Bacteria

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1992 Jul 1

PMID 1629329

Citations 7

Authors

L Selan

F Berlutti

C Passariello

M C Thaller

G Renzini

Affiliations

Soon will be listed here.

Abstract

The reliability of bioluminescence assays which employ the luciferin-luciferase ATP-dependent reaction to evaluate bacterial counts was studied, both in vitro and on urine specimens. Bioluminescence and cultural results for the most common urinary tract pathogens were analyzed. Furthermore, the influence of the culture medium, of the assaying method, and of the phase of growth on bioluminescence readings was studied. Results show that Proteus, Providencia, and Morganella strains are not correctly detected, neither in vitro nor in urine samples, by the standard assaying method. The analysis of assaying parameters demonstrated that some modifications to the extraction procedure of bacterial ATP could improve the reliability of this technique.

Citing Articles

Comparison of coliform paper test and ATP bioluminescence assay for monitoring the disinfection of kitchen utensils in canteens of hebei, China.

Tang Y, Sun J, Dong D, Zhang X, Jia R, Wang Y Heliyon. 2023; 9(4):e14839.

PMID: 37025863 PMC: 10070920. DOI: 10.1016/j.heliyon.2023.e14839.

Recent Methods for the Viability Assessment of Bacterial Pathogens: Advances, Challenges, and Future Perspectives.

Trinh K, Lee N Pathogens. 2022; 11(9).

PMID: 36145489 PMC: 9500772. DOI: 10.3390/pathogens11091057.

An Intact Cell Bioluminescence-Based Assay for the Simple and Rapid Diagnosis of Urinary Tract Infection.

Reyes S, Le N, Fuentes M, Upegui J, Dikici E, Broyles D Int J Mol Sci. 2020; 21(14).

PMID: 32708609 PMC: 7404122. DOI: 10.3390/ijms21145015.

Schrödinger's microbes: Tools for distinguishing the living from the dead in microbial ecosystems.

Emerson J, Adams R, Roman C, Brooks B, Coil D, Dahlhausen K Microbiome. 2017; 5(1):86.

PMID: 28810907 PMC: 5558654. DOI: 10.1186/s40168-017-0285-3.

Rapid antimicrobial susceptibility determination of uropathogens in clinical urine specimens by use of ATP bioluminescence.

Ivancic V, Mastali M, Percy N, Gornbein J, Babbitt J, Li Y J Clin Microbiol. 2008; 46(4):1213-9.

PMID: 18272708 PMC: 2292911. DOI: 10.1128/JCM.02036-07.

References

Thaller M, Berlutti F, Dainelli B, Pezzi R . New plate medium for screening and presumptive identification of gram-negative urinary tract pathogens. J Clin Microbiol. 1988; 26(4):791-3. PMC: 266453. DOI: 10.1128/jcm.26.4.791-793.1988. View

Drow D, Baum C, Hirschfield G . Comparison of the Lumac and Monolight systems for detection of bacteriuria by bioluminescence. J Clin Microbiol. 1984; 20(4):797-801. PMC: 271433. DOI: 10.1128/jcm.20.4.797-801.1984. View

Kolbeck J, Padgett R, Estevez E, Harrell L . Bioluminescence screening for bacteriuria. J Clin Microbiol. 1985; 21(4):527-30. PMC: 271712. DOI: 10.1128/jcm.21.4.527-530.1985. View

Ruokonen A, Koskinen M, Leinonen M, TIILIKAINEN A, Vihko R . Screening of bacteria in urine using luciferin-luciferase assay of microbial ATP: a comparative study. Ann Clin Biochem. 1982; 19(6):416-20. DOI: 10.1177/000456328201900605. View

Nichols W, CURTIS G, Johnston H . Analysis of the disagreement between automated bioluminescence-based and culture methods for detecting significant bacteriuria, with proposals for standardizing evaluations of bacteriuria detection methods. J Clin Microbiol. 1982; 15(5):802-9. PMC: 272193. DOI: 10.1128/jcm.15.5.802-809.1982. View

Beckers B, Lang H . Rapid detection of bacteriuria in bacteriological routine laboratory: comparison between bioluminescence method and culture techniques. Zentralbl Bakteriol Mikrobiol Hyg A Med Mikrobiol Infekt Parasitol. 1983; 254(4):515-20. View

Johnston H, Mitchell C, CURTIS G . An automated test for the detection of significant bacteriuria. Lancet. 1976; 2(7982):400-2. DOI: 10.1016/s0140-6736(76)92411-9. View

CONN R, CHARACHE P, Chappelle E . Limits of applicability of the firefly luminescence ATP assay for the detection of bacteria in clinical specimens. Am J Clin Pathol. 1975; 63(4):493-501. DOI: 10.1093/ajcp/63.4.493. View

Thore A, Ansehn S, Lundin A, Bergman S . Detection of bacteriuria by luciferase assay of adenosine triphosphate. J Clin Microbiol. 1975; 1(1):1-8. PMC: 274930. DOI: 10.1128/jcm.1.1.1-8.1975. View

10.

Welch W, Layman M, Southern Jr P . Evaluation of the MS-2 and Lumac systems for the rapid screening of urine specimens. Am J Clin Pathol. 1984; 81(5):629-33. DOI: 10.1093/ajcp/81.5.629. View

11.

McWalter P, Sharp C . Evaluation of a commercially available semi-automated bioluminescence system for bacteriuria screening. Eur J Clin Microbiol. 1982; 1(4):223-7. DOI: 10.1007/BF02019712. View

12.

Mackett D, Bascomb S, Easmon C . Evaluation of the Lumac kit for the detection of bacteriuria by bioluminescence. J Clin Pathol. 1982; 35(1):107-10. PMC: 497457. DOI: 10.1136/jcp.35.1.107. View

13.

Alexander D, EDERER G, Matsen J . Evaluation of an adenosine 5'-triphosphate assay as a screening method to detect significant bacteriuria. J Clin Microbiol. 1976; 3(1):42-6. PMC: 274223. DOI: 10.1128/jcm.3.1.42-46.1976. View