Hemolysin Plasmid Coding for the Virulence of a Nephropathogenic Escherichia Coli Strain

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1982 Jan 1

PMID 7033139

Citations 26

Authors

C Waalwijk

J F van den Bosch

D M MACLAREN

J de Graaff

Affiliations

Soon will be listed here.

Abstract

The nephropathogenic Escherichia coli strain P673 was shown to harbor two plasmids with molecular sizes of 70 and 41 megadaltons, respectively. The 70-megadalton plasmid, pCW1, coded for tetracycline resistance, whereas hemolysin production was coded by the 41-megadalton plasmid, pCW2. Plasmid pCW1 proved to be self-transmissible, in contrast to pCW2. Transfer of the hemolysin character was associated with the appearance of a 110-megadalton plasmid, pCW3. The incompatibility of pCW3 with both native plasmids and restriction enzyme analysis led to the conclusion that pCW3 is a cointegrate of pCW1 and pCW2, pCW2, carrying the hemolytic determinant, is involved in the nephropathogenic character of strain P673, because (i) elimination of pCW2 from P673 was associated with a loss of virulence and (ii) the nephropathogenicity of the avirulent mutant could be restored by reintroduction of pCW2 DNA as part of a cointegrate structure.

Citing Articles

Characterization of a hemolysin gene ytjA from Bacillus subtilis.

Liu J, Fang C, Jiang Y, Yan R Curr Microbiol. 2009; 58(6):642-7.

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Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli.

Berger H, Hacker J, Juarez A, Hughes C, Goebel W J Bacteriol. 1982; 152(3):1241-7.

PMID: 6754701 PMC: 221631. DOI: 10.1128/jb.152.3.1241-1247.1982.

Effect of Escherichia coli alpha-hemolysin on human peripheral leukocyte function in vitro.

Cavalieri S, Snyder I Infect Immun. 1982; 37(3):966-74.

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Characterization of Lactobacillus sp. strain 100-37 from the murine gastrointestinal tract: ecology, plasmid content, and antagonistic activity toward Clostridium ramosum H1.

McCormick E, Savage D Appl Environ Microbiol. 1983; 46(5):1103-12.

PMID: 6651295 PMC: 239526. DOI: 10.1128/aem.46.5.1103-1112.1983.

Escherichia coli alpha-hemolysin: characteristics and probable role in pathogenicity.

Cavalieri S, Bohach G, Snyder I Microbiol Rev. 1984; 48(4):326-43.

PMID: 6394977 PMC: 373222. DOI: 10.1128/mr.48.4.326-343.1984.

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