Virulence of Escherichia Coli in Experimental Hematogenous Pyelonephritis in Mice

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1979 Jul 1

PMID 383622

Citations 21

Authors

J F van den Bosch

J de Graaff

D M MACLAREN

Affiliations

Soon will be listed here.

Abstract

Differences in nephropathogenicity between Escherichia coli strains were studied by following the kinetics of the viable count in the mouse kidney during 8 h after intravenous injection. Assuming as a reference point that at zero time 0.1% of the inoculum was lodged in the kidney, we found that strains fell into three main groups with different behavior patterns: in group I, the viable count fell and remained low; in group II, the viable count first fell and then rose after 4 h, reaching the level of the reference point within 8 h; in group III, the viable count rose rapidly and remained high. The kinetics of the viable counts were also studied in blood, spleen, and liver: group I and II strains behaved similarly; only in the kidney did group II strains show higher counts than group I strains. These data suggest that group II strains are specifically virulent for the mouse kidney. Group III strains also gave higher viable counts in blood and spleen but comparable counts in the liver, suggesting that group III strains are more generally virulent. Fifty percent lethal dose measurements confirmed the conclusion that group I strains are avirulent and group III strains are the most virulent. Possible relationships between behavior pattern and serotype are discussed.

Citing Articles

Urinary virulence of Proteus mirabilis in two experimental mouse models.

Peerbooms P, Marian A, Verweij J, MACLAREN D Infect Immun. 1982; 36(3):1246-8.

PMID: 7047396 PMC: 551464. DOI: 10.1128/iai.36.3.1246-1248.1982.

Virulence of urinary and faecal Escherichia coli in relation to serotype, haemolysis and haemagglutination.

van den Bosch J, Postma P, Koopman P, de Graaff J, MACLAREN D, van Brenk D J Hyg (Lond). 1982; 88(3):567-77.

PMID: 7045221 PMC: 2134108. DOI: 10.1017/s002217240007042x.

Hemolysin plasmid coding for the virulence of a nephropathogenic Escherichia coli strain.

Waalwijk C, van den Bosch J, MACLAREN D, de Graaff J Infect Immun. 1982; 35(1):32-7.

PMID: 7033139 PMC: 350991. DOI: 10.1128/iai.35.1.32-37.1982.

Virulence of Escherichia coli strains isolated from urine of patients with acute cystitis and from faeces of healthy women.

van den Bosch J, Postma P, van Brenk D, GUINEE P, de Graaff J, MACLAREN D Antonie Van Leeuwenhoek. 1981; 47(2):97-106.

PMID: 7020589 DOI: 10.1007/BF02342193.

Evaluation of in vivo susceptibility of bacteria to antibiotics.

Thonus I Antonie Van Leeuwenhoek. 1981; 47(1):73-4.

PMID: 7018396 DOI: 10.1007/BF00399068.

References

Gorrill R . THE FATE OF PSEUDOMONAS AERUGINOSA, PROTEUS MIRABILIS AND ESCHERICHIA COLI IN THE MOUSE KIDNEY. J Pathol Bacteriol. 1965; 89:81-8. DOI: 10.1002/path.1700890110. View

KIMBALL H, Turck M, Petersdorf R . THE EPIDEMIOLOGY OF NONENTERIC ESCHERICHIA COLI INFECTIONS. III. RELATIONSHIP OF E. COLI ANTIGENS TO EXPERIMENTAL PYELONEPHRITIS. J Lab Clin Med. 1964; 63:907-13. View

KIMBALL H, Garcia M, Petersdorf R . THE EPIDEMIOLOGY OF NONENTERIC ESCHERICHIA COLI INFECTIONS. II. RELATIONSHIP OF PREVALENCE OF E. COLI IN URINARY INFECTIONS TO THE BACTERICIDAL EFFECT OF HUMAN SERUM. J Lab Clin Med. 1964; 63:901-6. View

Eden C, Hanson L, Jodal U, Lindberg U, Akerlund A . Variable adherence to normal human urinary-tract epithelial cells of Escherichia coli strains associated with various forms of urinary-tract infection. Lancet. 1976; 1(7984):490-2. View

Kaijser B, Hanson L, Jodal U, Robbins J . Frequency of E. coli K antigens in urinary-tract infections in children. Lancet. 1977; 1(8013):663-6. DOI: 10.1016/s0140-6736(77)92111-0. View

Bettelheim K . The sources of "OH" serotypes of Escherichia coli. J Hyg (Lond). 1978; 80(1):83-113. PMC: 2129983. DOI: 10.1017/s0022172400053420. View

ORSKOV I, ORSKOV F, JANN B, Jann K . Serology, chemistry, and genetics of O and K antigens of Escherichia coli. Bacteriol Rev. 1977; 41(3):667-710. PMC: 414020. DOI: 10.1128/br.41.3.667-710.1977. View

Wong W, Bettelheim K . Serotypes of Escherichia coli from urinary tract infections in Hong Kong. Zentralbl Bakteriol Orig A. 1976; 236(4):481-6. View

Guze L, Montgomerie J, Potter C, KALMANSON G . Pyelonephritis XVI. correlates of parasite virulence in acute ascending Escherichia coli pyelonephritis in mice undergoing diuresis. Yale J Biol Med. 1973; 46(3):203-11. PMC: 2591987. View

10.

Brumfitt W, Faiers M, Reeves D, Datta N . Antibiotic-resistant Escherichia coli causing urinary-tract infection in general practice: relation to faecal flora. Lancet. 1971; 1(7694):315-7. DOI: 10.1016/s0140-6736(71)91043-9. View

11.

Mabeck C, ORSKOV F, ORSKOV I . Escherichia coli serotypes and renal involvement in urinary-tract infection. Lancet. 1971; 1(7713):1312-4. DOI: 10.1016/s0140-6736(71)91884-8. View

12.

GLYNN A, Brumfitt W, Howard C . K antigens of Escherichia coli and renal involvement in urinary-tract infections. Lancet. 1971; 1(7698):514-6. View

13.

Spencer A, Mulcahy D, Shooter R, OGRADY F, Bettleheim K, Taylor J . Escherichia coli serotypes in urinary-tract infection in a medical ward. Lancet. 1968; 2(7573):839-42. DOI: 10.1016/s0140-6736(68)90998-7. View

14.

KALMANSON G, Harwick H, Turck M, Guze L . Urinary-tract infection: localisation and virulence of Escherichia coli. Lancet. 1975; 1(7899):134-6. DOI: 10.1016/s0140-6736(75)91430-0. View

15.

Kaijser B . Immunology of Escherichia coli: K antigen and its relation to urinary-tract infection. J Infect Dis. 1973; 127(6):670-7. DOI: 10.1093/infdis/127.6.670. View

16.

Harle E, BULLEN J, Thomson D . Influence of oestrogen on experimental pyelonephritis caused by Escherichia coli. Lancet. 1975; 2(7929):283-6. DOI: 10.1016/s0140-6736(75)92726-9. View

17.

MACLAREN D . Letter: Pyelonephritis and coliform infections of the urinary tract. Lancet. 1975; 2(7934):556. DOI: 10.1016/s0140-6736(75)90933-2. View

18.

Taylor P, Roberts A, Gower P . Evaluation of a technique for the estimation of serum bactericidal activity against Gram-negative organisms. Med Lab Technol. 1972; 29(3):272-9. View

19.

FRIED F, VERMEULEN C, Ginsburg M, Cone C . Etiology of pyelonephritis: further evidence associating the production of experimental pyelonephritis with hemolysis in Escherichia coli. J Urol. 1971; 106(3):351-4. DOI: 10.1016/s0022-5347(17)61286-2. View

20.

Bettelheim K, Taylor J . A study of Escherichia coli isolated from chronic urinary infection. J Med Microbiol. 1969; 2(3):225-36. DOI: 10.1099/00222615-2-3-225. View