Haemophilus Influenzae Can Use Human Transferrin As a Sole Source for Required Iron

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1985 Apr 1

PMID 3872264

Citations 40

Authors

D A Herrington

P F Sparling

Affiliations

Soon will be listed here.

Abstract

Haemophilus influenzae grown on enriched medium containing protoporphyrin IX rather than hemin was iron starved by the addition of the chelator ethylenediamine di-o-hydroxyphenylacetic acid. Iron starvation could be overcome in each of 33 H. influenzae type b isolates by 30% Fe-saturated human transferrin but not by human lactoferrin. Among nontypeable H. influenzae, 28 of 35 isolates, including 2 of 3 systemic isolates, were able to utilize Fe-transferrin. None of 18 H. parainfluenzae isolates was able to use Fe-transferrin. Iron starvation of H. influenzae type b resulted in increased amounts of three membrane proteins of 94,000 to 98,000 daltons.

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