Amber-mutants of Plasmid Mini-F Defective in Replication

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 7003308

Citations 12

Authors

R Eichelaub

H Wehlmann

Affiliations

Soon will be listed here.

Abstract

Amber mutants of the mini-F plasmid pML31 were isolated with the mutagen hydroxylamine. Under non-permissive conditions amber mutants segregate and show no incorporation of label into supercoiled plasmid DNA in double-label experiments. Wild-type and one mutant of mini-F were integrated by recombinant DNA techniques into the single EcoRI site of plasmid vector pBR322. Plasmid specific proteins were labeled in minicells and analysed by SDS-PAGE. A 34,000 dalton molecular weight protein was identified to be missing in the amber mutant of plasmid mini-F.

Citing Articles

Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

Komai N, Nishizawa T, Hayakawa Y, Murotsu T, Matsubara K Mol Gen Genet. 1982; 186(2):193-203.

PMID: 7050629 DOI: 10.1007/BF00331850.

Identification of the pifC gene and its role in negative control of F factor pif gene expression.

Miller J, MALAMY M J Bacteriol. 1983; 156(1):338-47.

PMID: 6413493 PMC: 215087. DOI: 10.1128/jb.156.1.338-347.1983.

An essential gene for replication of the mini-F plasmid from origin I.

Tanimoto K, Iino T Mol Gen Genet. 1984; 196(1):59-63.

PMID: 6384731 DOI: 10.1007/BF00334092.

An amber replication mutant of F plasmid mapped in the minimal replication region.

Maki S, Kuribayashi M, Miki T, Horiuchi T Mol Gen Genet. 1983; 191(2):231-7.

PMID: 6353162 DOI: 10.1007/BF00334819.

Novobiocin-induced elimination of F'lac and mini-F plasmids from Escherichia coli.

Wolfson J, Hooper D, SWARTZ M, Swartz M, McHugh G J Bacteriol. 1983; 156(3):1165-70.

PMID: 6315678 PMC: 217963. DOI: 10.1128/jb.156.3.1165-1170.1983.

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