Bidirection Replication from a Unique Origin in a Mini-F Plasmid

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1977 Mar 1

PMID 322144

Citations 45

Authors

R Eichenlaub

D Figurski

D R Helinski

Affiliations

Soon will be listed here.

Abstract

Replicating molecules of the mini F-kanamycin resistance plasmid, pML31, derived from F'lac, have been isolated from Escherichia coli as covalently-closed circular DNA molecules. These molecules were examined in the electron microscope after digestion with either EcoRI or BamHI restriction endonuclease. The structure of the majority of the molecules replicating was consistent with a bidirectional mode of replication starting at a unique origin on the F-fragment. This origin is located approximately 2.3 kilobases from one of the EcoRI sites. Orientation of the F-fragment relative to the physical map of F showed the position of the origin to be at 42.6 kilobases. A small proportion of molecules appeared to be replicating unidirectionally in either direction from this origin. Termination of replication of pML31 apparently occurs in the fragment containing the locus for kanamycin resistance in a unique region opposite the origin in the circular DNA molecule.

Citing Articles

Order from clutter: selective interactions at mammalian replication origins.

Aladjem M, Redon C Nat Rev Genet. 2016; 18(2):101-116.

PMID: 27867195 PMC: 6596300. DOI: 10.1038/nrg.2016.141.

CDI Systems Are Stably Maintained by a Cell-Contact Mediated Surveillance Mechanism.

Ruhe Z, Nguyen J, Chen A, Leung N, Hayes C, Low D PLoS Genet. 2016; 12(6):e1006145.

PMID: 27355474 PMC: 4927057. DOI: 10.1371/journal.pgen.1006145.

Detection and mapping of six miniF-encoded proteins by cloning analysis of dissected miniF segments.

Komai N, Nishizawa T, Hayakawa Y, Murotsu T, Matsubara K Mol Gen Genet. 1982; 186(2):193-203.

PMID: 7050629 DOI: 10.1007/BF00331850.

Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC.

Meijer M, Messer W J Bacteriol. 1980; 143(2):1049-53.

PMID: 7009545 PMC: 294416. DOI: 10.1128/jb.143.2.1049-1053.1980.

Plasmid mini-F encoded proteins.

Wehlmann H, Eichenlaub R Mol Gen Genet. 1980; 180(1):205-11.

PMID: 7003309 DOI: 10.1007/BF00267371.

References

Palchaudhuri S, Maas W . Physical mapping of a DNA sequence common to plasmids of incompatibility group F I. Proc Natl Acad Sci U S A. 1977; 74(3):1190-4. PMC: 430648. DOI: 10.1073/pnas.74.3.1190. View

Hershfield V, Boyer H, Yanofsky C, Lovett M, Helinski D . Plasmid ColEl as a molecular vehicle for cloning and amplification of DNA. Proc Natl Acad Sci U S A. 1974; 71(9):3455-9. PMC: 433792. DOI: 10.1073/pnas.71.9.3455. View

Masters M, Broda P . Evidence for the bidirectional replications of the Escherichia coli chromosome. Nat New Biol. 1971; 232(31):137-40. DOI: 10.1038/newbio232137a0. View

Jaenisch R, Mayer A, Levine A . Replicating SV40 molecules containing closed circular template DNA strands. Nat New Biol. 1971; 233(37):72-5. DOI: 10.1038/newbio233072a0. View

BOURGAUX P, BOURGAUX-RAMOISY D . Unwinding of replicating polyoma virus DNA. J Mol Biol. 1972; 70(3):399-413. DOI: 10.1016/0022-2836(72)90548-7. View

SEBRING E, Kelly Jr T, THOREN M, Salzman N . Structure of replicating simian virus 40 deoxyribonucleic acid molecules. J Virol. 1971; 8(4):478-90. PMC: 376221. DOI: 10.1128/JVI.8.4.478-490.1971. View

Lovett M, Katz L, Helinski D . Unidirectional replication of plasmid ColE1 DNA. Nature. 1974; 251(5473):337-40. DOI: 10.1038/251337a0. View

Gyurasits E, Wake R . Bidirectional chromosome replication in Bacillus subtilis. J Mol Biol. 1973; 73(1):55-63. DOI: 10.1016/0022-2836(73)90158-7. View

Guerry P, Leblanc D, Falkow S . General method for the isolation of plasmid deoxyribonucleic acid. J Bacteriol. 1973; 116(2):1064-6. PMC: 285495. DOI: 10.1128/jb.116.2.1064-1066.1973. View

10.

Sharp P, Hsu M, Otsubo E, Davidson N . Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors. J Mol Biol. 1972; 71(2):471-97. DOI: 10.1016/0022-2836(72)90363-4. View

11.

Cohen S, Chang A, Hsu L . Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci U S A. 1972; 69(8):2110-4. PMC: 426879. DOI: 10.1073/pnas.69.8.2110. View

12.

Nishioka Y, EISENSTARK A . Sequence of genes replicated in Salmonella typhimurium as examined by transduction techniques. J Bacteriol. 1970; 102(2):320-33. PMC: 247554. DOI: 10.1128/jb.102.2.320-333.1970. View

13.

Bazaral M, Helinski D . Characterization of multiple circular DNA forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968; 7(10):3513-20. DOI: 10.1021/bi00850a028. View

14.

Guyer M, Figurski D, Davidson N . Electron microscope study of a plasmid chimera containing the replication region of the Escherichia coli F plasmid. J Bacteriol. 1976; 127(2):988-97. PMC: 233009. DOI: 10.1128/jb.127.2.988-997.1976. View

15.

Lovett M, Helinski D . Method for the isolation of the replication region of a bacterial replicon: construction of a mini-F'kn plasmid. J Bacteriol. 1976; 127(2):982-7. PMC: 233008. DOI: 10.1128/jb.127.2.982-987.1976. View

16.

Lovett M, Sparks R, Helinski D . Bidirectional replication of plasmid R6K DNA in Escherichia coli; correspondence between origin of replication and position of single-strand break in relaxed complex. Proc Natl Acad Sci U S A. 1975; 72(8):2905-9. PMC: 432887. DOI: 10.1073/pnas.72.8.2905. View

17.

Cabello F, Timmis K, Cohen S . Replication control in a composite plasmid constructed by in vitro linkage of two distinct replicons. Nature. 1976; 259(5541):285-90. DOI: 10.1038/259285a0. View

18.

Wilson G, Young F . Isolation of a sequence-specific endonuclease (BamI) from Bacillus amyloliquefaciens H. J Mol Biol. 1975; 97(1):123-5. DOI: 10.1016/s0022-2836(75)80028-3. View

19.

Schnos M, Inman R . Position of branch points in replicating lambda DNA. J Mol Biol. 1970; 51(1):61-73. DOI: 10.1016/0022-2836(70)90270-6. View